Phenotype Image Detail

Image

Caption

Embryonic development of Cripto^tm1.1Gmin/Cripto^tm1.1Gmin (F78A), and Cripto^tm1Eda/Cripto^tm1Eda (KO) mutants at head-fold stage. Wild-type (top), Cripto^tm1.1Gmin/Cripto^tm1.1Gmin (F78A, middle), and Cripto^tm1Eda/Cripto^tm1Eda (KO, bottom) embryos at 8.5 dpc stained by whole mount mRNA in situ hybridization. All mutant embryos are shown with anterior side toward the left. (A, A', and A") Double staining for Brachyury (blue) and Otx2 (red). Brachyury, which identifies posterior mesoderm in wild-type embryos (A), is expressed in the posterior side of Cripto^tm1.1Gmin/Cripto^tm1.1Gmin (F78A) opposite from the Otx2 expression domain, revealing the presence of posterior mesoderm (A'). In contrast, Brachyury expression is absent in the Cripto^tm1Eda/Cripto^tm1Eda (KO) mutant (A"). (B, B', and B") Double staining for Krox20 (blue) and Otx2 (red). Krox20, which identifies rhombomers three and five in wild-type embryos (B) marks two parallel stripes of cells located in the posterior region of the Cripto^tm1.1Gmin/Cripto^tm1.1Gmin (F78A) embryo (B'), whereas it is absent in the Cripto^tm1Eda/Cripto^tm1Eda (KO) mutant (B"). (C, C', and C") Mox1 expression identifies paraxial mesoderm in wild-type embryos (C). Mox1 mRNA is detected in a small stripe of cells located in the posterior region of the Cripto^tm1.1Gmin/Cripto^tm1.1Gmin (F78A) embryo (C'), whereas it is absent in the Cripto^tm1Eda/Cripto^tm1Eda (KO) mutant (C"). Bars, 100 um.

Copyright

This image is from D'Andrea D, J Cell Biol 2008 Feb 11;180(3):597-605, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:186907

Associated Alleles

Symbol	Name
Cripto^tm1.1Gmin	cripto, EGF-CFC family member; targeted mutation 1.1, Gabriella Minchiotti
Cripto^tm1Eda	cripto, EGF-CFC family member; targeted mutation 1, Eileen D Adamson

Associated Genotypes

Allelic Composition	Genetic Background
Cripto^tm1.1Gmin/Cripto^tm1.1Gmin	involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Cripto^tm1Eda/Cripto^tm1Eda	either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * Black Swiss * C57BL/6)