Phenotype Image Detail

Image

Caption

Neurodegeneration in Vps18^tm1.1Wtao/Vps18^tm1.1Wtao Tg(Nes-cre)1Kln/0 (CKO) brains. (A-D) Histological analyses of the hippocampus and cerebellum of the Vps18 CKO mice. (A and B) Disruption of the morphological structures of hippocampus in the Vps18 CKO mice at P10 due to significant cell loss. Arrow and arrowhead in (A) indicate the split and neuron-loosely-associated CA3 region separately. (C and D) Less foliation and a smaller size of the Vps18 CKO cerebellum. Sagittal cryosections of indicated mouse brains at P1 (A and C) or P10 (B and D) were stained with H&E and microphotographed. (E) Dramatic loss of the Purkinje cells in the Vps18^tm1.1Wtao/Vps18^tm1.1Wtao Tg(Pcp2-cre)2Mpin/0 (Vps18F/F; Pcp2-Cre) mice. The cryosections of indicated mouse cerebellums were stained with anti-calbindin. (F) Western blot analysis of total proteins from P4 brains discloses activation of caspase-3. Blot shown is representative of three experiments with similar results. (G-I) A striking increase of GFAP signals in cerebral cortex (G), hippocampus (H) and cerebellum (I) of P10 Vps18 CKO mouse brain. (J-L) Increased apoptotic cells in the Vps18 CKO brains. Cryosections of the cerebral cortex (J), hippocampus (K) and cerebellum (L) from indicated mice at P10 were stained with anti-caspase-3. All images are representative brain sections from at least three mice per group. [Scale bar: 200 um (A-D); 100 um (E, G-L).]

Copyright

This image is from Peng C, J Biol Chem 2012 Aug 1;(): and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:186307

Associated Alleles

Symbol	Name
Tg(Nes-cre)1Kln	transgene insertion 1, Rudiger Klein
Tg(Pcp2-cre)2Mpin	transgene insertion 2, Max-Planck-Institute of Neurobiology
Vps18^tm1.1Wtao	VPS18 CORVET/HOPS core subunit; targeted mutation 1.1, Wufan Tao

Associated Genotypes

Allelic Composition	Genetic Background
Vps18^tm1.1Wtao/Vps18^tm1.1Wtao Tg(Nes-cre)1Kln/0	involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6 * SJL
Vps18^tm1.1Wtao/Vps18^tm1.1Wtao Tg(Pcp2-cre)2Mpin/0	B.129-Vps18^tm1.1Wtao Tg(Pcp2-cre)2Mpin