Phenotype Image Detail

Image

Caption

Accumulation of autophagosomes, late endosomes and amorphous densely stained vesicles in Vps18^tm1.1Wtao/Vps18^tm1.1Wtao Tg(Nes-cre)1Kln/0 (CKO) brains. (A and B) Typical electron micrographs of Ctrl or Vps18 CKO cortical neuron at P10. (C and D) Enlarged view of the cropped region in (A) and (B). Arrowheads: early autophagosomes; Double arrows: late autophagosomes; arrows: multivesicular bodies. N and M in (A-C) represent nucleus and mitochondria respectively. (E) Bar graph shows great accumulation of early and late autophagosomes and mutivesicular bodies in the Vps18 CKO cerebral cortex. The organelles in thirteen randomly chosen neurons on electronic microscope photographs from two sets of mouse brains were counted. (F) Electron microscopy analysis reveals that enormously swollen dystrophic neurites in the Vps18 CKO hippocampus are full of amorphous, multilaminar body-like vesicles. Images are representative mouse brain sections from at least three mice per group. Values in (E) represent the means +/- SEM. **P value < 0.01. [Scale bar: 2 um (A, B and F); 500 nm (C and D)]

Copyright

This image is from Peng C, J Biol Chem 2012 Aug 1;(): and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:186307

Associated Alleles

Symbol	Name
Tg(Nes-cre)1Kln	transgene insertion 1, Rudiger Klein
Vps18^tm1.1Wtao	VPS18 CORVET/HOPS core subunit; targeted mutation 1.1, Wufan Tao

Associated Genotypes

Allelic Composition	Genetic Background
Vps18^tm1.1Wtao/Vps18^tm1.1Wtao Tg(Nes-cre)1Kln/0	involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6 * SJL