Phenotype Image Detail

Image

Caption

H&E-stained cross-sections of the skin and ears. A) Normal skin at 10 weeks of age from Supv3l1^tm2Jkl/Supv3l1⁺ Tg(Mx1-cre)1Cgn/0 (Mx1-Cre Supv3L1^tm2Jkl/+) mouse. Ad, adipose layer; Der, dermis; Eder, epidermis; SC, subcutaneous muscle fibers. B) Skin from an age-matched Supv3l1^tm2Jkl/Supv3l1^tm2Jkl Tg(Mx1-cre)1Cgn/0 (Mx1-Cre Supv3L1^{tm2Jkl/tm2Jkl}) animal. HC, hyperkeratosis. Note the thickened epidermis, severe hyperkeratosis, dystrophic dermis, lack of sebaceous glands, and almost total absence of adipose and muscle layers. C) Higher-power magnification of the skin from an Supv3l1^tm2Jkl/Supv3l1^tm2Jkl Tg(Mx1-cre)1Cgn/0 (Mx1-Cre Supv3L1^{tm2Jkl/tm2Jkl}) animal showing parakeratosis (arrow) and scaling. D) Cross-section of a normal ear of an Supv3l1^tm2Jkl/Supv3l1⁺ Tg(Mx1-cre)1Cgn/0 (Mx1-Cre Supv3L1^tm2Jkl/+) animal at 10 weeks of age. E) Cross-section of an ear of an age-matched Supv3l1^tm2Jkl/Supv3l1^tm2Jkl Tg(Mx1-cre)1Cgn/0 (Mx1-Cre Supv3L1^{tm2Jkl/tm2Jkl}) animal showing disfigured morphology, thickened epidermis, hyperkeratosis, and neutrophilic microabscesses (arrowhead). F) Another Supv3l1^tm2Jkl/Supv3l1^tm2Jkl Tg(Mx1-cre)1Cgn/0 (Mx1-Cre Supv3L1^{tm2Jkl/tm2Jkl}) cross-section showing an hyperkeratotic area with absent epidermal cells (arrowhead). G) H&E-stained skin section of an Supv3l1^tm2Jkl/Supv3l1^tm2Jkl Tg(CAG-cre/Esr1*)5Amc/0 (Actb-Esr1/Cre Supv3L1^{tm2Jkl/tm2Jkl}) animal at the age of 12 weeks. H) Dorsal skin from an Supv3l1^tm2Jkl/Supv3l1^tm2Jkl Tg(CAG-cre/Esr1*)5Amc/0 (Actb-Esr1/Cre Supv3L1^{tm2Jkl/tm2Jkl}) animal injected subcutaneously with tamoxifen at the age of 25 days. Note the absence of adipose tissue, paucity of subcutaneous muscle, dilated blood vessels, and mild hyperkeratosis. I) Skin from the control animal Supv3l1^tm2Jkl/Supv3l1⁺ Tg(CAG-cre/Esr1*)5Amc/0 (Actb-Esr1/Cre Supv3L1^tm2Jkl/+) topically treated with 4-hydroxy tamoxifen. J) A skin sample from an Supv3l1^tm2Jkl/Supv3l1^tm2Jkl Tg(CAG-cre/Esr1*)5Amc/0 (Actb-Esr1/Cre Supv3L1^{tm2Jkl/tm2Jkl}) animal after topical 4-hydroxy tamoxifen treatment. Note hyperkeratotic and parakeratotic changes along with a significantly thickened epidermal layer of cells. Dilated blood vessels in the dermal layer are marked with asterisks. K) Ear cross-section of a control Supv3l1^tm2.1Jkl/Supv3l1⁺ Tg(KRT14-cre/ERT)20Efu/0 (KRT14-Cre/Esr1 Supv3L1^tm3Jkl/+) mouse after subcutaneous administration of tamoxifen. Biopsy was taken 4 weeks after subcutaneous administration was completed. L) Ear cross-section of a Supv3l1^tm2.1Jkl/Supv3l1^tm2.2Jkl Tg(KRT14-cre/ERT)20Efu/0 (KRT14-Cre/Esr1 Supv3L1^{tm3Jkl/tm4Jkl}) littermate of the same sex and age after subcutaneous administration of tamoxifen. Note atrophy of hair follicles, sweat glands, thickening of epidermis, hyperkeratosis, dilation of blood vessels, and infiltrative changes.

Copyright

This image is from Paul E, Mamm Genome 2009 Feb;20(2):92-108 and is displayed with the permission of Springer Science + Business Media, Inc., New York who owns the copyright. J:144991

Associated Alleles

Symbol	Name
Supv3l1^tm2.1Jkl	suppressor of var1, 3-like 1 (S. cerevisiae); targeted mutation 2.1, Jan Klysik
Supv3l1^tm2.2Jkl	suppressor of var1, 3-like 1 (S. cerevisiae); targeted mutation 2.2, Jan Klysik
Supv3l1^tm2Jkl	suppressor of var1, 3-like 1 (S. cerevisiae); targeted mutation 2, Jan Klysik
Tg(CAG-cre/Esr1*)5Amc	transgene insertion 5, Andrew P McMahon
Tg(KRT14-cre/ERT)20Efu	transgene insertion 20, Elaine Fuchs
Tg(Mx1-cre)1Cgn	transgene insertion 1, University of Cologne

Associated Genotypes

Allelic Composition	Genetic Background
Supv3l1^tm2Jkl/Supv3l1^tm2Jkl Tg(Mx1-cre)1Cgn/0	involves: 129P2/OlaHsd * C57BL/6 * CBA
Supv3l1^tm2Jkl/Supv3l1^tm2Jkl Tg(CAG-cre/Esr1*)5Amc/0	involves: 129P2/OlaHsd * C57BL/6
Supv3l1^tm2.1Jkl/Supv3l1^tm2.2Jkl Tg(KRT14-cre/ERT)20Efu/0	involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6 * CD-1 * FVB/N