Phenotype Image Detail

Image

Caption

Chromosome stability in Rev3l-deficient B cells. (A) Chromosome aberrations in B cells of Rev3l^tm1Rsky/Rev3l^tm1.1Rsky Tg(Cr2-cre)3Cgn/0 (Polzeta^f/delta/CD21-cre) mice. B cells of Rev3l^tm1Rsky/Rev3l^tm1.1Rsky Tg(Cr2-cre)3Cgn/0 mice and control mice were activated to proliferate by the stimulation with LPS + IL-4 or LPS alone for 3 days. After induction of chromosome condensation and fixation of the cells, metaphase spreads were prepared and stained with DAPI for the visualization by fluorescence microscopy. Shown are the combined data of two mice per genotype. Bar, approximately 5 um. (B) Spectral karyotyping (SKY) of B cells of Rev3l^tm1Rsky/Rev3l^tm1.1Rsky Tg(Cr2-cre)3Cgn/0 mice. After stimulation of B cells with LPS + IL-4, metaphases were prepared, hybridized with suitable probes, and examined by fluorescence microscopy. Shown are the combined data of two mice per genotype. Red arrows indicate chromosome abnormalities. Bar, approximately 5 um.

Copyright

This image is from Schenten D, J Exp Med 2009 Feb 16;206(2):477-90, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:146453

Associated Alleles

Symbol	Name
Rev3l^tm1.1Rsky	REV3 like, DNA directed polymerase zeta catalytic subunit; targeted mutation 1.1, Klaus Rajewsky
Rev3l^tm1Rsky	REV3 like, DNA directed polymerase zeta catalytic subunit; targeted mutation 1, Klaus Rajewsky
Tg(Cr2-cre)3Cgn	transgene insertion 3, University of Cologne

Associated Genotypes

Allelic Composition	Genetic Background
Rev3l^tm1Rsky/Rev3l^tm1.1Rsky Tg(Cr2-cre)3Cgn/?	involves: C57BL/6