Phenotype Image Detail

Image

Caption

A, histology of blood, spleen, and liver, from Hbb^{tm1.1(HBG1,HBB*)Ryan}/Hbb⁺ (human gamma beta ⁰ globin KI) mice shows Beta thalassemic phenotypes. Wright-Giemsa stained peripheral blood smears of wild-type control and heterozygous mutant mice. Typical red blood cell targeting, hypochromia, and anisopoikilocytosis are observed in mutant mice. The normal red (erythroid) and white (lymphoid) pulp sections seen in the wild-type spleen were replaced by an expanded erythroid compartment in the mutant mice compared with the wild-type control. Livers of thalassemic mutant mice contain clusters of erythroid cells indicative of extramedullary hematopoiesis. Image magnifications are shown in parentheses. Slides were analyzed on Nikon Eclipse E800 or TE2000 microscopes (Nikon, Tokyo, Japan). Image magnifications are shown in parentheses. B, electron microscopy of circulating red blood cells from wild-type and mutant adult mice demonstrating alpha globin chain inclusions (scale bar, 1 um). C, comparison of spleen size as a percentage of body weight of wild type and mutant mice show a significant splenomegaly in the thalassemic mice (n >/= 8 in each group).

Copyright

This image is from Huo Y, J Biol Chem 2009 Feb 20;284(8):4889-96 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:147906

Associated Alleles

Symbol	Name
Hbb^tm1.1(HBG1,HBB*)Ryan	hemoglobin beta chain complex; targeted mutation 1.1, Thomas M Ryan

Associated Genotypes

Allelic Composition	Genetic Background
Hbb^tm1.1(HBG1,HBB*)Ryan/Hbb^+	involves: C57BL/6J