Phenotype Image Detail

Image

Caption

Increase of tubular cell proliferation and apoptosis in the cystic kidneys of Tg(CAG-PKD2)#Hwl/? (TG) mice. PCNA-positive cells in the tubules of the cortex and medulla were significantly increased in PKD2 transgenic (mutant) mice. Specifically, considerable numbers of PCNA-positive cells were also cyst-lining cells, and were observed in surrounding regions. A, a and c, kidney of a wild-type mouse. b and d, kidney of a mutant mouse. Boxed regions are magnified panels c and d. Note that flattened cells in the cyst were stained with PCNA. B, quantification of PCNA positive cells in wild-type (WT) and mutant (TG) kidneys, showing that the number of PCNA-positive cells per tubule was significantly increased in the mutant kidney compared with that of wild-type mice. The apoptotic index was evaluated by TUNEL assay. The number of apoptotic cells in the cysts and tubules around cysts were significantly increased in the mutant mouse compared with that of wild-type mice. C, a and c, kidney of a wild-type mouse. b and d, kidney of a mutant mouse. Boxed regions were magnified (c and d). Note that, similar to cell proliferation, apoptotic cells were enriched in cyst-lining cuboidal cells. D, quantification of TUNEL positive cells.

Copyright

This image is from Park EY, J Biol Chem 2009 Mar 13;284(11):7214-22 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:148377

Associated Alleles

Symbol	Name
Tg(CAG-PKD2)#Hwl	transgene insertion, Han-Woong Lee

Associated Genotypes

Allelic Composition	Genetic Background
Tg(CAG-PKD2)#Hwl/?	involves: FVB/NJ