Phenotype Image Detail

Image

Caption

Epidermal and hair follicle (HF) phenotype in Itgb1^tm1Ref/Itgb1^tm5.1Ref Tg(KRT5-cre)5132Jlj/0 (hpm) mice. (A, B) Haematoxylin-eosin stained sections from the back skin of 14-day-old control, hpm and Itgb1^tm1Ref/Itgb1^tm1Ref Tg(KRT5-cre)5132Jlj/0 (K5-b1) mice. (A) hpm mice displayed stunted HF morphogenesis leading to two HF types (black triangle - fully developed with multilayered outer root sheath (ORS); black square - shortened, malformed and prematurely arrested in morphogenesis), while in K5-beta1 mice all HFs were severely distorted. (B) Control epidermis consists of a monolayer of cuboidal cells firmly attached to the dermis, while hpm epidermis consisted of 2-3 keratinocyte layers with few microblisters at the BM zone (arrows). The K5-beta1 epidermis consisted of 2-7 layers of roundish, polygonal or flattened keratinocytes that were frequently detached from the dermis forming large blisters (arrows). (C, D) Haematoxylin-eosin stained sections from the back skin of 6.5-month-old control and hpm and of 5-week-old K5-beta1 mice. (C) Progressive hair loss in hpm epidermis is accompanied by the development of dermal fibrosis with scattered melanin deposits (arrowheads). The remaining HFs are severely malformed. In K5-beta1 mice the dermal fibrosis developed already at 5 weeks of age and was accompanied by an almost complete loss of HFs and large melanin deposition in the dermis (arrowheads). (D) The epidermis of the back skin of 6.5-month-old hpm mice is hyperthickened but has almost no microblisters. The epidermis of 5-week-old K5-beta1 mice is severely hyperthickened and shows reduced blistering when compared to 2-week-old skin. (E,F) Immunostaining for LM-332 of back skin sections. (E) At 14 days of age LM-332 (red) is diffusely distributed at the dermal-epidermal junction of the hpm mouse skin, however less pronounced than in K5-beta1 epidermis. Nuclei were stained with DAPI (blue). (F) At 6.5 months of age LM-332 (red) shows a largely normal, linear deposition at the dermal-epidermal junction of hpm skin. LM-332 deposition is also improved in 5-week-old K5-beta1 epidermis. Nuclei were stained with DAPI (blue).

Copyright

This image is from Piwko-Czuchra A, PLoS ONE 2009;4(5):e5488, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:148885

Associated Alleles

Symbol	Name
Itgb1^tm1Ref	integrin beta 1 (fibronectin receptor beta); targeted mutation 1, Reinhard Fassler
Itgb1^tm5.1Ref	integrin beta 1 (fibronectin receptor beta); targeted mutation 5.1, Reinhard Fassler
Tg(KRT5-cre)5132Jlj	transgene insertion 5132, Jose Luis Jorcano

Associated Genotypes

Allelic Composition	Genetic Background
Itgb1^tm1Ref/Itgb1^tm5.1Ref Tg(KRT5-cre)5132Jlj/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * DBA/2J
Itgb1^tm1Ref/Itgb1^tm1Ref Tg(KRT5-cre)5132Jlj/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * DBA/2J