Phenotype Image Detail

Image

Caption

Reduced spreading defect of keratinocytes from old Itgb1^tm1Ref/Itgb1^tm5.1Ref Tg(KRT5-cre)5132Jlj/0 (hpm) mice. (A) Freshly isolated keratinocytes from 2.5-month-old mice seeded on a mixture of collagen 1 (Col1) and fibronectin (FN). In contrast to control cells, hpm cells neither spread nor grow ex vivo. Time points after seeding are indicated. (B) Proliferation rate of keratinocytes from 3-week-old mice cultured for 2 days on Col1/FN and pulse-labelled with BrdU for 10 or 24 hours. Compared to control cells, hpm keratinocytes have significantly reduced proliferation rate. Bars represent data from keratinocytes of 3 mice per genotype. Error bars indicate s.d. (C) Cell adhesion of hpm keratinocytes from 2.5-month-old mice plated on Col1/FN or on LM-332 is similar to control cells. 5 and 4 independent experiments were performed on Col1/FN or LM-332, respectively. Error bars indicate s.d. (D) Keratinocytes from 2.5-month-old control and hpm mice were cultured for 2 days on Col1/FN and immunostained for beta1 integrin, paxillin and vinculin (red), and F-actin (phalloidin; green). Nuclei were stained with DAPI (blue). hpm cells failed to develop focal adhesions and to organize the actin cytoskeleton into stress fibers.

Copyright

This image is from Piwko-Czuchra A, PLoS ONE 2009;4(5):e5488, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:148885

Associated Alleles

Symbol	Name
Itgb1^tm1Ref	integrin beta 1 (fibronectin receptor beta); targeted mutation 1, Reinhard Fassler
Itgb1^tm5.1Ref	integrin beta 1 (fibronectin receptor beta); targeted mutation 5.1, Reinhard Fassler
Tg(KRT5-cre)5132Jlj	transgene insertion 5132, Jose Luis Jorcano

Associated Genotypes

Allelic Composition	Genetic Background
Itgb1^tm1Ref/Itgb1^tm5.1Ref Tg(KRT5-cre)5132Jlj/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * DBA/2J