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Caption | Reduced spreading defect of keratinocytes from old Itgb1tm1Ref/Itgb1tm5.1Ref Tg(KRT5-cre)5132Jlj/0 (hpm) mice. (A) Freshly isolated keratinocytes from 2.5-month-old mice seeded on a mixture of collagen 1 (Col1) and fibronectin (FN). In contrast to control cells, hpm cells neither spread nor grow ex vivo. Time points after seeding are indicated. (B) Proliferation rate of keratinocytes from 3-week-old mice cultured for 2 days on Col1/FN and pulse-labelled with BrdU for 10 or 24 hours. Compared to control cells, hpm keratinocytes have significantly reduced proliferation rate. Bars represent data from keratinocytes of 3 mice per genotype. Error bars indicate s.d. (C) Cell adhesion of hpm keratinocytes from 2.5-month-old mice plated on Col1/FN or on LM-332 is similar to control cells. 5 and 4 independent experiments were performed on Col1/FN or LM-332, respectively. Error bars indicate s.d. (D) Keratinocytes from 2.5-month-old control and hpm mice were cultured for 2 days on Col1/FN and immunostained for beta1 integrin, paxillin and vinculin (red), and F-actin (phalloidin; green). Nuclei were stained with DAPI (blue). hpm cells failed to develop focal adhesions and to organize the actin cytoskeleton into stress fibers. | ||||||||
Copyright | This image is from Piwko-Czuchra A, PLoS ONE 2009;4(5):e5488, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:148885 | ||||||||
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Associated Genotypes |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 08/02/2024 MGI 6.24 |
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