Phenotype Image Detail

Image

Caption

Primary fibroblasts were processed for immunofluorescence on experimental day 5, as shown in Fig. 2A. (A) Example of metaphase in a control cell (NuMA +/+ ) and two Numa1^tm1.1Dwc/Numa1^tm1.1Dwc Tg(CAG-cre/Esr1*)5Amc/0 (NuMA delta22/delta22 ) primary fibroblasts. Arrows indicate centrosomes. (B) Anaphase in wild-type and two mutant primary cells. Each image represents a maximum intensity projection of a deconvolved series of z sections spanning the entire cell in 0.2-um intervals. Arrows indicate centrosomes. Tubulin is shown in green, and phosphorylated histone H3 is shown in purple.

Copyright

This image is from Silk AD, J Cell Biol 2009 Mar 9;184(5):677-90, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:146214

Associated Alleles

Symbol	Name
Numa1^tm1.1Dwc	nuclear mitotic apparatus protein 1; targeted mutation 1.1, Don W Cleveland
Tg(CAG-cre/Esr1*)5Amc	transgene insertion 5, Andrew P McMahon

Associated Genotypes

Allelic Composition	Genetic Background
Numa1^tm1.1Dwc/Numa1^tm1.1Dwc Tg(CAG-cre/Esr1*)5Amc/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA * SJL