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Caption | (A) Distance between sister kinetochores in cells arrested in metaphase with MG132 and incubated on ice for 10 min to selectively depolymerize nonkinetochore fiber microtubules. Images represent maximum intensity projections of a deconvolved series of z sections spanning the entire cell in 0.2 um intervals (projection) or single deconvolved z sections. Kinetochore pairs were identified in single z sections by the releative positioning of kinetochores and orientation of associated kinetochore fibers. Blue, DNA; green, microtubules; red, kinetochores. Bars: (left) 5 um; and (right) 2.5 um. (B) Interkinetochore distances of paired sister chromatids in Numa1tm1.1Dwc/Numa1+ Tg(CAG-cre/Esr1*)5Amc/0 (NuMA +/delta22), Numa1tm1.1Dwc/Numa1tm1.1Dwc Tg(CAG-cre/Esr1*)5Amc/0 (NuMA delta 22/delta22), and nocodazole (Noc)-treated control cells. The boxes represent the interquartile (middle 50%), and the whiskers represent the full range. Horizontal lines represent the median value. (C) Examples of heterozygous cells with fully aligned chromosomes and homozygous mutant fibroblasts with chromosome alignment defects. Cells were treated as in A and processed for immunofluorescence to visualize DNA (purple) and tubulin (green). Bars, 5 um. (D) Percentage of spindles showing chromosome alignments defects. ***, P < 0.0001. Error bars indicate SEM. | ||||||
Copyright | This image is from Silk AD, J Cell Biol 2009 Mar 9;184(5):677-90, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:146214 | ||||||
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Associated Genotypes |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 08/21/2024 MGI 6.24 |
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