Phenotype Image Detail

Image

Caption

(A) Distance between sister kinetochores in cells arrested in metaphase with MG132 and incubated on ice for 10 min to selectively depolymerize nonkinetochore fiber microtubules. Images represent maximum intensity projections of a deconvolved series of z sections spanning the entire cell in 0.2 um intervals (projection) or single deconvolved z sections. Kinetochore pairs were identified in single z sections by the releative positioning of kinetochores and orientation of associated kinetochore fibers. Blue, DNA; green, microtubules; red, kinetochores. Bars: (left) 5 um; and (right) 2.5 um. (B) Interkinetochore distances of paired sister chromatids in Numa1^tm1.1Dwc/Numa1⁺ Tg(CAG-cre/Esr1*)5Amc/0 (NuMA +/delta22), Numa1^tm1.1Dwc/Numa1^tm1.1Dwc Tg(CAG-cre/Esr1*)5Amc/0 (NuMA delta 22/delta22), and nocodazole (Noc)-treated control cells. The boxes represent the interquartile (middle 50%), and the whiskers represent the full range. Horizontal lines represent the median value. (C) Examples of heterozygous cells with fully aligned chromosomes and homozygous mutant fibroblasts with chromosome alignment defects. Cells were treated as in A and processed for immunofluorescence to visualize DNA (purple) and tubulin (green). Bars, 5 um. (D) Percentage of spindles showing chromosome alignments defects. ***, P < 0.0001. Error bars indicate SEM.

Copyright

This image is from Silk AD, J Cell Biol 2009 Mar 9;184(5):677-90, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:146214

Associated Alleles

Symbol	Name
Numa1^tm1.1Dwc	nuclear mitotic apparatus protein 1; targeted mutation 1.1, Don W Cleveland
Tg(CAG-cre/Esr1*)5Amc	transgene insertion 5, Andrew P McMahon

Associated Genotypes

Allelic Composition	Genetic Background
Numa1^tm1.1Dwc/Numa1^tm1.1Dwc Tg(CAG-cre/Esr1*)5Amc/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA * SJL