Phenotype Image Detail

Image

Caption

Uterine fibrosis in aged nulliparous Antxr2^tm1.1Jkit/Antxr2^tm1.1Jkit (Antxr2-/-) mice is accompanied by atypical vasculature and inflammation. (A) CD31 immunostaining (brown color) of three-month-old reproductive tracts reveal atypical/open blood vessels (arrows) throughout the homozygous mutant uterus and cervix. Boxed areas are blown up to highlight vasculature. Ep, endometrial epithelium; CM, circular myometrium; LM, longitudinal myometrium. Scale bars on uterus photos, 200 um. Scale bars on cervix photos, 150 um. (B) Coimmunofluoresence for blood endothelial cell marker, endomucin (green color), and lymphatic endothelial cell marker, lyve-1 (red color) on three-month-old uterine tissue. DAPI (blue color) is used for nuclear staining. Lymphatic vessels (arrowheads) in homozygous mutant tissue are enlarged. Scale bar, 100 um. (C) Immunofluorescent staining for macrophage marker, F4/80 (red color), revealed an increased inflammatory response in three-month-old and ten-month-old homozygous mutant uterine tissue. DAPI (blue color) is used for nuclear staining. Scale bars, 200 um.

Copyright

This image is from Reeves CV, PLoS One 2012;7(4):e34862, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:187210

Associated Alleles

Symbol	Name
Antxr2^tm1.1Jkit	anthrax toxin receptor 2; targeted mutation 1.1, Jan K Kitajewski

Associated Genotypes

Allelic Composition	Genetic Background
Antxr2^tm1.1Jkit/Antxr2^tm1.1Jkit	involves: 129 * C57BL/6