Phenotype Image Detail

Image

Caption

Increased collagen and fibronectin content in aged nulliparous Antxr2^tm1.1Jkit/Antxr2^tm1.1Jkit (Antxr2-/-) uterine tissue. (A) Immunofluorescent staining of uterine tissue isolated from six-month-old mice demonstrated increased type I collagen, type IV collagen and fibronectin deposition in the homozygous mutant tissue. DAPI (blue) was used for nuclear staining. Negative controls demonstrate specificity of the antibodies. Ep, endometrial epithelium; S, stroma; M, myometrium; GE, glandular epithelium. Scale bars, 150 um. (B) Uterine lysates from six-month-old mice, wild-type (Antxr2+/+, n = 2) and mutant (n = 2), were immunoblotted for type I collagen (precursor type I collagen indicated by arrow and mature type I collagen indicated by arrowhead), type VI collagen and fibronectin. Alpha tubulin is shown as a loading control. (C) Densitometric analysis of blots in panel B presented as relative levels of designated ECM protein normalized to respective alpha tubulin. The mean +/- the standard deviation are represented, * = P<0.05.

Copyright

This image is from Reeves CV, PLoS One 2012;7(4):e34862, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:187210

Associated Alleles

Symbol	Name
Antxr2^tm1.1Jkit	anthrax toxin receptor 2; targeted mutation 1.1, Jan K Kitajewski

Associated Genotypes

Allelic Composition	Genetic Background
Antxr2^tm1.1Jkit/Antxr2^tm1.1Jkit	involves: 129 * C57BL/6