Phenotype Image Detail

Image

Caption

Recently weaned mice were injected 3 consecutive days with tamoxifen and the small intestines from Slc39a4^tm2Gka/Slc39a4^tm2Gka Tg(Vil1-cre/ERT2)23Syr/0 (Zip4- intestine knockout; Zip4 intest KO) and control (Con) mice (5 mice per group) were harvested on the indicated days after initiation of the knockout. (A) Paraffin sections of small intestine were processed for IHC using an antibody against mouse Sox9. Brown color indicates positive immunostaining. Bars below the d2 and d6 panels of mutant section demarcate the approximate length of the crypts in each panel and black arrowheads indicate Sox9 negative cells interspersed among the positive cells (d2) and Sox9 negative cells at the base of the crypts (d6). (B) Frozen sections of small intestine from mutant mice were incubated with ZP1 and photographed using the GFP cube. Brightness of the sections was reduced equally in Photoshop. Green indicates positive signal for zinc-induced ZP1 fluorescence. A higher power view of the red boxed region in the d0 panel is shown below and white arrows demarcate labeled Paneth cells. (C) Paraffin sections were processed for IHC using an antibody against mouse Sox9 or lysozyme. Brown color indicates positive immunostaining. Serial sections were also stained with PAS to detected mucin. Dark pink to red color indicates positive PAS staining. Black arrows demarcate Paneth cells in the d6 Sox9 stained panel. Tamoxifen injections did not affect Sox9, lysozyme or mucin staining of small intestine sections from control littermates (not shown).

Copyright

This image is from Geiser J, PLoS Genet 2012 Jun;8(6):e1002766, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:187178

Associated Alleles

Symbol	Name
Slc39a4^tm2Gka	solute carrier family 39 (zinc transporter), member 4; targeted mutation 2, Glen K Andrews
Tg(Vil1-cre/ERT2)23Syr	transgene insertion 23, Sylvie Robine

Associated Genotypes

Allelic Composition	Genetic Background
Slc39a4^tm2Gka/Slc39a4^tm2Gka Tg(Vil1-cre/ERT2)23Syr/0	involves: 129P2/OlaHsd * C57BL/6 * DBA/2