Phenotype Image Detail

Image

Caption

Abnormal axial skeleton formation in E12.5 Apc^tm1Rsmi/Apc^tm1Rsmi Tg(Col2a1-cre)1Rsjo/0 (Col2a1-Cre;Apc^15lox/15lox) embryos is already detectable at E12.5. (A) Immunostaining for beta-catenin combined with Alcian blue (AB) staining and (B-E) gene expression analysis by in situ hybridization with indicated probes on consecutive transversal sections of the sclerotome of a mutant embryo and control littermate at E12.5. (F-J) Similiar analysis of vertebrae primordia at E14.6. Beta-catenin-positive spindle-shaped cells lacked expression of all indicated chondrogenic and osteogenic markers (arrowheads). Scale bars: 100 um.

Copyright

This image is from Miclea RL, BMC Dev Biol 2009;9():26, an open-access article, licensee BioMed Central Ltd. J:149225

Associated Alleles

Symbol	Name
Apc^tm1Rsmi	APC, WNT signaling pathway regulator; targeted mutation 1, Ron Smits
Tg(Col2a1-cre)1Rsjo	transgene insertion 1, Randall S Johnson

Associated Genotypes

Allelic Composition	Genetic Background
Apc^tm1Rsmi/Apc^tm1Rsmi Tg(Col2a1-cre)1Rsjo/0	involves: 129P2/OlaHsd * FVB/N