Phenotype Image Detail

Image

Caption

Figure 3. Delayed onset to puberty and ocular, endocrine, haematological, and plasma abnormalities. A. Periodic acid-Schiff (PAS) staining and cleaved (activated) caspase-3 immunostaining of adrenal sections from 16 week-old virgin female Cenpj^tm/tm mice (n = 3) confirmed corticomedullary pigmentation and ongoing apoptosis in the X-zone, respectively (representative images, scale bars 100 microm). B. Breeding records of Cenpj^tm/tm females set up with Cenpj^tm/tm males at 6-7 weeks of age showed that Cenpj^tm/tm females produce their first litter around four weeks later than Cenpj^+/+ females. *P = 0.012, t-test.C. Top panel shows normal cornea from a Cenpj^+/+ mouse. Cenpj^tm/tm mice had disruption of the Descemet's membrane and corneal endothelium (arrow). Middle panel shows normal anterior segment from a Cenpj^+/+ mouse. The angle was displaced anteriorly in eyes from Cenpj^tm/tm mice and ciliary process morphology was abnormal. (a, angle; i, iris; cb, ciliary body; l, lens). Bottom panel shows normal retina from a Cenpj^+/+ mouse eye. The retina photoreceptor cells of Cenpj^tm/tm mice were reduced in number and showed columnar disorganized (arrow). (ONL, outer nuclear layer). D. Immunohistochemical staining for Cenpj in Cenpj^+/+ embryo eye (E14.5; RNL retinal neuroblast layer). E. Intra-peritoneal glucose tolerance test to show that female Cenpj^tm/tm mice have a 15 minute delay in response to glucose challenge (n = 4 Cenpj^tm/tm vs. n = 32 Cenpj^+/+, *P = 2[?]10-5, t-test). Graph also shows n = 9 Cenpjtm/+ and n = 670 baseline wildtype controls. F. Plasma albumin levels were decreased in Cenpj^tmm males (n = 8 Cenpj^tm/tm vs. n = 35 Cenpj^+/+, *P = 4.9[?]10-5, t-test). Graph also shows n = 7 Cenpj^tm/+ and n = 768 baseline wildtype controls. G. Flow cytometric analysis of peripheral blood leukocytes in Cenpj mice revealed an increase in the number of CD8+CD3+ and H. total CD3+ cells. Data shows total counts per 30 000 propidium-iodide (PI) negative, CD45-positive cells from male mice. For n = 9 Cenpj^tm/tm vs. n = 30 Cenpj^+/+: CD3+CD8+ *P = 0.0002 and CD3 *P = 2.9x10-5, Mann-Whitney-Wilcoxon test. Graphs also show n = 7 Cenpj^+/tm and n = 356 baseline wildtype controls. For all 'Box and Whisker' plots, the lower whisker extends to the lowest datum still within 1.5 Inter-quartile range (IQR) of the lower quartile. The upper whisker extends to the highest datum still within 1.5 IQR of the upper quartile.

Copyright

This image is from McIntyre RE, PLoS Genet 2012;8(11):e1003022, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:194085

Associated Alleles

Symbol	Name
Cenpj^tm1a(EUCOMM)Wtsi	centromere protein J; targeted mutation 1a, Wellcome Trust Sanger Institute
Cenpj^tm1a(EUCOMM)Wtsi	centromere protein J; targeted mutation 1a, Wellcome Trust Sanger Institute

Associated Genotypes

Allelic Composition	Genetic Background
Cenpj^tm1a(EUCOMM)Wtsi/Cenpj^tm1a(EUCOMM)Wtsi	B6Brd;B6N-Tyr^c-Brd Cenpj^tm1a(EUCOMM)Wtsi/Wtsi