Phenotype Image Detail

Image

Caption

A, Oil Red O staining of heart cryosections of wild-type (WT; Plin5^+/+) (panels a, b, e and f) and Plin5^tm1Tosu/Plin5^tm1Tosu (Plin5^-/-) (panels c, d, g and h) mice in the fed (panels a-d) and fasted (panels e-h) states. Panels a, c, e and g, low magnification (bar, 25 um). Panels b, d, f and h, high magnification (bar, 10 um). No lipid droplets (LDs) were observed even by close inspection of the sections of mutant mice in either state. In WT mice, out of 20 randomly selected microscopic fields (each 86.4 X 60.5 um), four in the fed state and all in the fasted state contained detectable LDs. The number of LDs in a field was 18.7 +/- 11.0 in the fed mice and 966 +/- 409 in the fasted mice, respectively (p = 4.3 x 10⁹). The sizes of LDs were 0.22 +/- 0.04 um in the fed and 0.64 +/- 0.11 um in the fasted states, respectively (p = 2.3 x 10^-7). B, representative electron microscopic images of the hearts of WT and mutant mice fasted overnight. Left panels, low magnification (bar, 10 um), and right panels, high magnification (bar, 2 um). Note that LDs are observed as white ovals only in WT mice. C, Oil Red O staining of soleus muscle cryosections. Panels a and b, WT, and panels c and d, mutant mice, both fasted overnight. Panels a and c, low magnification. Bar, 100 um. Panels b and d, high magnification of the area enclosed by a rectangle. Bar, 25 um.

Copyright

This image is from Kuramoto K, J Biol Chem 2012 Jul 6;287(28):23852-63 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:188390

Associated Alleles

Symbol	Name
Plin5^tm1Tosu	perilipin 5; targeted mutation 1, Takashi Osumi

Associated Genotypes

Allelic Composition	Genetic Background
Plin5^tm1Tosu/Plin5^tm1Tosu	involves: C57BL/6JJcl