Phenotype Image Detail

Image

Caption

Constitutive deletion of exons 2-4 of Rspo3 is embryonic lethal during E10. Images show pairs of freshly dissected, littermate embryos that were later genotyped by PCR. (a) A control E10.5 embryo shows normal forelimb (arrow) and heart (clear arrowhead) morphology, whereas a Rspo3^tm1.2Jcob/Rspo3^tm1.2Jcob (Rspo3-/-) embryo (b) has obvious vascular defects, including hemorrhaging (black arrowhead) and a swollen pericardial sac (clear arrowhead). The small forelimb bud (arrow) is characteristic of an early E10 embryo. (c, d) Control and mutant E10.75 embryos within yolk sac membranes. The control membrane shows obvious vascularization (arrows) while the mutant is pale (arrow) without visible blood vessels. (e, f) The E10.75 embryos from (c) to (d) after removal of embryonic membranes. The mutant (f) has an engorged pericardial sac (clear arrowhead) and small forelimb buds (arrow) and has the overall size of an early E10 embryo. A weak heartbeat was detected in this embryo. (g) A control embryo shows normal E11.5 morphology. A mutant littermate (h) is dead and shows developmental arrest at early E10, as judged by embryo size and forelimb bud morphology (arrow) (compare to (b, f)). All mutant embryos (14 of 14) dissected at E11.5 were dead at a stage similar to that in (h). All images are at the same magnification; scale bar = 1mm.

Copyright

This image is from Neufeld S, Genesis 2012 Oct;50(10):741-9, and is displayed with the permission of Wiley-Blackwell, who owns the Copyright. J:188812

Associated Alleles

Symbol	Name
Rspo3^tm1.2Jcob	R-spondin 3; targeted mutation 1.2, John Cobb

Associated Genotypes

Allelic Composition	Genetic Background
Rspo3^tm1.2Jcob/Rspo3^tm1.2Jcob	involves: 129S6/SvEvTac * C57BL/6 * CD-1 * SJL