Phenotype Image Detail

Image

Caption

Appearance of limbs in newborn animals; f, forelimbs; arrows, hindlimbs. (a) Typical appearance of wild-type limbs. (b) Common digit defects in the hindlimbs of an Rspo2^ftls/Rspo2^ftls (Rspo2^Tg/Tg) mutant (arrows). This animal illustrates the most common category of defects in animals with this genotype where autopods are present but malformed (a similar phenotype was seen in 12 of 19 mutants). (c) Tg(Prrx1-cre)1Cjt/0 Rspo3^tm1.1Jcob/Rspo3^tm1.2Jcob Rspo2^ftls/Rspo2^ftls (Prx1-Cre; Rspo3^F/-; Rspo2^Tg/Tg) double mutant appears to have no hindlimbs extending from the body (arrow). A skeletal preparation from this animal revealed the presence of femora but no distal hindlimb elements (shown in Fig. 5e). Rspo2^ftls/Rspo2^ftls animals die at birth due to a lung defect, which is why there is no milk in the stomachs of pups in (b) and (c). Some mutant progeny were likely never recovered at birth due to this neonatal lethality.

Copyright

This image is from Neufeld S, Genesis 2012 Oct;50(10):741-9, and is displayed with the permission of Wiley-Blackwell, who owns the Copyright. J:188812

Associated Alleles

Symbol	Name
Rspo2^ftls	R-spondin 2; footless
Rspo3^tm1.2Jcob	R-spondin 3; targeted mutation 1.2, John Cobb
Tg(Prrx1-cre)1Cjt	transgene insertion 1, Clifford J Tabin

Associated Genotypes

Allelic Composition	Genetic Background
Rspo2^ftls/Rspo2^ftls Rspo3^tm1.1Jcob/Rspo3^tm1.2Jcob Tg(Prrx1-cre)1Cjt/0	involves: 129S6/SvEvTac * C57BL/6 * CD-1 * SJL
Rspo2^ftls/Rspo2^ftls	Not Specified