Phenotype Image Detail

Image

Caption

Induction of neuroinflammation by doxycycline-mediated suppression of transgenic Hexb. CD68 staining (brown DAB staining) shows activated microglia in the thalamus (A-H). In animals heterozygous for Hexb^tm1Rlp (A and E), limited CD68 staining was present. Hexb^tm1Rlp/Hexb^tm1Rlp (Hexb-/-) animals (B and F) had large amoeboid microglia that stained for CD68 in the presence or absence of doxycycline (Dox). In Sandhoff animals (Hexb^tm1Rlp/Hexb^tm1Rlp Tg(Hexb-tTA2S,tetO-Hexb)#Tjsa/0 (Hexb-/-Hex^Tg) or Hexb^tm1Rlp/Hexb^tm1Rlp Tg(SYN1-tTA2S,tetO-Hexb)#Tjsa/0 (Hexb-/-SYN^Tg)), no neuroinflammation is presence in the absence of doxycycline (C and D). However, in the presence of doxycycline, animals developed marked microgliosis (G and H) similiar to Sandhoff animals at their humane endpoint. Comparable results were observed with GFAP staining for astrocytes (I-P). Scale bar = 50 um.

Copyright

This image is from Sargeant TJ, PLoS Genet 2012 Sep;8(9):e1002943, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:190450

Associated Alleles

Symbol	Name
Hexb^tm1Rlp	hexosaminidase B; targeted mutation 1, Richard L Proia
Tg(SYN1-tTA2S,tetO-Hexb)#Tjsa	transgene insertion, Timothy J Sargeant

Associated Genotypes

Allelic Composition	Genetic Background
Hexb^tm1Rlp/Hexb^tm1Rlp Tg(Hexb-tTA2S,tetO-Hexb)#Tjsa/0	involves: 129S4/SvJae * C57BL/6 * CBA
Hexb^tm1Rlp/Hexb^tm1Rlp Tg(SYN1-tTA2S,tetO-Hexb)#Tjsa/0	involves: 129S4/SvJae * C57BL/6 * CBA