Phenotype Image Detail

Image

Caption

(A-D) MITF expression at E12.5 for the indicated genotypes. The Vsx2^tm1.1Eml/Vsx2^tm1.1Eml (R227W) retina expressed MITF at much higher levels compared to the Vsx2^or-J/Vsx2^or-J (orJ) and Vsx2^tm1.1Itl/Vsx2^tm1.1Itl (R200Q) mutants. (D') Merged images of VSX2^[R227W] (red) and MITF (green) shows overlap in expression. The lack of VSX2^[R227W] expression in the peripheral retina corresponded to the highest levels of MITF. (E-H) OTX expression at E12.5 for the indicated genotypes. OTX expression was highest in the R227W retina. (H') Merged images of VSX2^[R227W] (red) and OTX (green). Like MITF, OTX expression was highest in regions lacking VSX2^[R227W]. MITF and OTX were also expressed in retinal pigment epithelium (RPE; outside lower dashed lines). (I-K) Relative mRNA expression levels of pan-Mitf (I), Otx1 and Otx2 (J), and the D-, H-, A-,J-, and B-Mitf isoforms (K) in E12.5 retinas of the indicated genotypes as determined by qRT-PCR. Samples were normalized to the expression level for each transcript in the orJ retina (white bars). *P<0.05 Scale bar: 50 um.

Copyright

This image is from Zou C, PLoS Genet 2012 Sep;8(9):e1002924, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:190452

Associated Alleles

Symbol	Name
Vsx2^or-J	visual system homeobox 2; ocular retardation Jackson
Vsx2^tm1.1Eml	visual system homeobox 2; targeted mutation 1.1, Edward M Levine
Vsx2^tm1.1Itl	visual system homeobox 2; targeted mutation 1.1, inGenious Targeting Laboratory

Associated Genotypes

Allelic Composition	Genetic Background
Vsx2^tm1.1Itl/Vsx2^tm1.1Itl	involves: 129S6/SvEvTac
Vsx2^tm1.1Eml/Vsx2^tm1.1Eml	involves: 129S1/Sv * 129X1/SvJ
Vsx2^or-J/Vsx2^or-J	involves: 129S1/Sv