Phenotype Image Detail

Image

Caption

(A-F) PITX2 (red) and MITF (green) expression at E10.5 (A-C) and E12.5 (D-F). Limited PITX2+ cells were detected in the vitreal chamber (between retina and lens) of wild-type eyes. PITX2+ cells were not detected in the vitreal chamber of Vsx2^or-J/Vsx2^or-J (orJ) and Vsx2^tm1.1Eml/Vsx2^tm1.1Eml (R227W) mutant eyes at E10.5, but were abundant by E12.5 and continuous with periocular mesenchyme (POM) at the retinal periphery (arrowheads). MITF expression levels were modestly upregulated at E10.5 in the mutant retinas and were clearly elevated by E12.5. (G) Relative expression levels of A-Mitf, H-Mitf, and pan-Mitf in E10.5 whole retina and lens explants cultured for 48 hr. R227W expression levels were normalized to orJ. Invasion of POM into the vitreal chamber did not occur in these cultures (data not shown). (H) Relative expression levels of A-Mitf, H-Mitf, and pan-Mitf in physically manipulated E10.5 R227W whole retina and lens explants cultured for 48 hr after the following manipulations: "-POM" (retina and lens only); "mock" (retina partially separated from lens); "+POM" (retina partially separated from lens with surrounding POM intact); "POM imp" (retina partially separated from lens and POM implanted into vitreal cavity). Expression levels were normalized to the "-POM" condition. *P<0.05; ** P<0.01 Scale bar: 50 um.

Copyright

This image is from Zou C, PLoS Genet 2012 Sep;8(9):e1002924, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:190452

Associated Alleles

Symbol	Name
Vsx2^or-J	visual system homeobox 2; ocular retardation Jackson
Vsx2^tm1.1Eml	visual system homeobox 2; targeted mutation 1.1, Edward M Levine

Associated Genotypes

Allelic Composition	Genetic Background
Vsx2^tm1.1Eml/Vsx2^tm1.1Eml	involves: 129S1/Sv * 129X1/SvJ
Vsx2^or-J/Vsx2^or-J	involves: 129S1/Sv