Phenotype Image Detail

Image

Caption

(e-h) These images depict glomeruli from six week old animals; sections were stained with hematoxylin (dark blue nuclei) and periodic acid Schiff (pink/red basement membranes and sclerotic solid areas). Note the structure of a control glomerulus (e), with intact capillary loops. (f-g) depict three different glomeruli from the kidney of a mouse with Fras1 deleted in podocytes, Fras1^bl/Fras1^tm1.2Pjsc; the glomerulus in f is relatively normal compared with disorganized and sclerotic glomeruli in g and h. (i, j) Electron microscope images of a control (i) and Fras1^bl/Fras1^tm1.2Pjsc (j) glomerulus. In the former, note the prominent Bowman's space (BS) around the podocytes (P), and the open capillary loops (the black asterisk indicates a red cell within a glomerular capillary). In contrast, the Fras1^bl/Fras1^tm1.2Pjsc (Fras1^bl/D2) glomerulus has less prominent capillary loops but a more prominent central mesangial core (white *). (k-o) Hematoxylin and periodic acid Schiff stained sections of kidneys from 12-week-old mice. A control glomerulus is depicted in (k). The range of phenotypes of glomeruli from a Fras1^bl/Fras1^tm1.2Pjsc mouse is shown in (l-o); note the sclerotic (solid pink) areas and, in (l and m), and the abnormal presence of red cells in Bowman's space. Scale bars: i and j, 5 um; c and d, 25 um; b-h and k-o, 100 um.

Copyright

This image is from Pitera JE, Genesis 2012 Dec;50(12):892-8, and is displayed with the permission of Wiley-Blackwell, who owns the Copyright. J:190659

Associated Alleles

Symbol	Name
Fras1^bl	Fraser extracellular matrix complex subunit 1; blebbed
Fras1^tm1.2Pjsc	Fraser extracellular matrix complex subunit 1; targeted mutation 1.2, Peter J Scambler

Associated Genotypes

Allelic Composition	Genetic Background
Fras1^bl/Fras1^tm1.2Pjsc	involves: 101/H * C3H/HeH * C57BL/6 * FVB/N * SJL