Phenotype Image Detail

Image

Caption

Six1 gene disruption perturbs myogenic differentiation of satellite cell (SC) descendants ex vivo. 1 week after tamoxifen (TM) treatment, extensor digitorum longus (EDL) myofibers from control and Six1^tm2.1Mair/Six1^tm2.1Mair Pax7^{tm1(cre/ERT2)Gaka}/Pax7⁺ (Six1KO) mice were plated on Matrigel, and cultures were analyzed after 6 and 9 days of culture ex vivo. (A) Myogenic cells grown for 6 days were immunolocalized for Desmin (myoblast marker) and myosin heavy chain (MyHC) (differentiation marker) proteins. (B) Mutant cells exhibit limited differentiation potential ex vivo compared with control cells. (C) Myogenic cells grown for 9 days were immunolocalized for Six1, Pax7 (undifferentiated state marker), and MyHC (differentiated state marker) proteins. (D) Mutant cells fuse less efficiently and form smaller myotubes compared with control cells.

Copyright

This image is from Le Grand F, J Cell Biol 2012 Sep 3;198(5):815-32, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:190460

Associated Alleles

Symbol	Name
Pax7^tm1(cre/ERT2)Gaka	paired box 7; targeted mutation 1, Gabrielle Kardon
Six1^tm2.1Mair	sine oculis-related homeobox 1; targeted mutation 2.1, Pascal Maire

Associated Genotypes

Allelic Composition	Genetic Background
Six1^tm2.1Mair/Six1^tm2.1Mair Pax7^tm1(cre/ERT2)Gaka/Pax7^+	involves: 129S6/SvEvTac * C57BL/6 * C57BL/6Cr * C57BL/6NTac