Phenotype Image Detail

Image

Caption

Six1 gene disruption perturbs myogenic differentiation of satellite cell (SC) descendants ex vivo. 1 week after tamoxifen (TM) treatment, extensor digitorum longus (EDL) myofibers from control and Six1^tm2.1Mair/Six1^tm2.1Mair Pax7^{tm1(cre/ERT2)Gaka}/Pax7⁺ (Six1KO) mice were plated on Matrigel, and cultures were analyzed after 6 and 9 days of culture ex vivo. (G) SC-derived myogenic cells grown for 9 days were immunolocalized for Pax7 and both MyoD and Ki67 proteins. (H) Mutant cells generate more "reserve" cells (Pax7⁺/MyoD^-/Ki67^-; arrows) compared with control cells. Error bars indicate standard deviations. *, P < 0.02. Bars, 10 um.

Copyright

This image is from Le Grand F, J Cell Biol 2012 Sep 3;198(5):815-32, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:190460

Associated Alleles

Symbol	Name
Pax7^tm1(cre/ERT2)Gaka	paired box 7; targeted mutation 1, Gabrielle Kardon
Six1^tm2.1Mair	sine oculis-related homeobox 1; targeted mutation 2.1, Pascal Maire

Associated Genotypes

Allelic Composition	Genetic Background
Six1^tm2.1Mair/Six1^tm2.1Mair Pax7^tm1(cre/ERT2)Gaka/Pax7^+	involves: 129S6/SvEvTac * C57BL/6 * C57BL/6Cr * C57BL/6NTac