Phenotype Image Detail

Image

Caption

Impairment of ossification in conditional Sp7^tm1Rnis/Sp7^tm1Rnis mice. A and B, Sp7^tm1Rnis/Sp7^tm1Rnis Tg(Prrx1-cre)1Cjt/0 (flox/flox; Prx1-Cre) mice. Skeletal preparation of forelimb stained with alcian blue and alizarin red at E15.5 days. Mutant and control littermate (flox/flox or flox/+) (A) are shown. HE staining of tibia of mutant and control littermate at E16.5 days are shown (B). C-E, Sp7^tm1Rnis/Sp7^tm1Rnis Tg(Col2a1-cre)1Bhr/0 (flox/flox;Col2-Cre) mice. Skeletal preparation of forelimb stained with alcian blue and alizarin red at E16.5 days. Mutant and control littermate are shown (C). HE staining of tibia of mutant and control littermate at E16.5 days (D). Von Kossa staining of tibia of mutant and control littermate at E16.5 (E). F, MMP13 immunostaining of tibia of mutant and control littermate at E16.5 days. G, Sp7^tm1Rnis/Sp7^tm1Rnis Tg(Col11a2-cre)1Ntsu/0 (flox/flox;Col11-Cre) mice. HE staining of tibia of mutant and control littermate at E16.5 days. Scale bar; 100 um.

Copyright

This image is from Nishimura R, J Biol Chem 2012 Sep 28;287(40):33179-90 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:191589

Associated Alleles

Symbol	Name
Sp7^tm1Rnis	Sp7 transcription factor 7; targeted mutation 1, Riko Nishimura
Tg(Col11a2-cre)1Ntsu	transgene insertion 1, Noriyuki Tsumaki
Tg(Col2a1-cre)1Bhr	transgene insertion 1, Richard R Behringer
Tg(Prrx1-cre)1Cjt	transgene insertion 1, Clifford J Tabin

Associated Genotypes

Allelic Composition	Genetic Background
Sp7^tm1Rnis/Sp7^tm1Rnis Tg(Col11a2-cre)1Ntsu/0	involves: C57BL * C57BL/6 * CBA/JNCrlj * DBA/2
Sp7^tm1Rnis/Sp7^tm1Rnis Tg(Col2a1-cre)1Bhr/0	involves: C57BL * C57BL/6 * CBA/JNCrlj * SJL
Sp7^tm1Rnis/Sp7^tm1Rnis Tg(Prrx1-cre)1Cjt/0	involves: C57BL * C57BL/6 * CBA/JNCrlj * SJL/J