Phenotype Image Detail

Image

Caption

Morphological and molecular changes in the synaptic ribbons of photoreceptors in Mirc35hg^{Gt(D016D06)Wrst}/Mirc35hg^{Gt(D016D06)Wrst} (miR-183C^GT/GT) mice. (A-D) Confocal images of Ribeye immunofluorescence (IF) in the retinas of 5-week- (A and B) and 1-year-old (C and D) mutant mice. (B and D) and their wild-type (WT, miR-183C+/+) littermate controls (A and C). (E and F) Coimmunostaining of Ribeye (green) and peanut agglutinin (PNA) (red) in retinas of 5-week-old mutant mice (F) and their WT littermate controls (E). (G) An electron micrograph of a ribbon synapse of a WT mouse, showing a typical triad configuration of photoreceptor ribbon synapse. SR, synaptic ribbon; H, horizontal cell; B, bipolar cell. (H) Example of a 3D reconstruction of synaptic ribbons in the outer plexiform layer (OPL) of mutant mice (n = 2) and their WT littermates (n = 2), respectively. (Scale cube, 500 nm³.) (I and J) Distribution of synaptic ribons by size (I) and mean ribbon areas of mutant mice (red; n = 72) and WT littermate controls (black; n = 49) (J). Data are shown as mean +/- SEM; *P < 0.01.

Copyright

This image is from Lumayag S, Proc Natl Acad Sci U S A 2013 Feb 5;110(6):E507-16. Copyright 2013 National Academy of Sciences, U.S.A. J:193822

Associated Alleles

Symbol	Name
Mirc35hg^Gt(D016D06)Wrst	microRNA cluster 35, host gene; gene trap D016D06, German Gene Trap Consortium

Associated Genotypes

Allelic Composition	Genetic Background
Mirc35hg^Gt(D016D06)Wrst/Mirc35hg^Gt(D016D06)Wrst	involves: 129S2/SvPas * C57BL/6J