Phenotype Image Detail

Image

Caption

Abnormal mitochondrial network in Afg3l2^tm1Arte/Afg3l2^tm1Arte Tg(Pcp2-cre)2Mpin/0 (Afg312^PC-KO) mice. Analyses were performed in animals of the shown genotypes crossed with Gt(ROSA)26Sor^tm1Lrsn/Gt(ROSA)26Sor⁺ mice. (A-D) The mitochondrial network is labeled with mt-YFP, (E and F) while Purkinje cell (PC) dendrites are decorated using an antibody against calbindin. (G and H) in merged images; pseudocolors were used; calbindin is in green, while mt-YFP is in red. Images in A and B show enlarged views of the boxes in C and D, respectively. (C) mt-YFP expression in control mice shows an elongated network of mitochondria (E and G) that follows the pattern of PC dendrites labeled with calbindin, (A) with mitochondria filling the entire space of large caliber dendrites. (D) In Afg3l2^tm1Arte/Afg3l2^tm1Arte Tg(Pcp2-cre)2Mpin/0 Gt(ROSA)26Sor^tm1Lrsn/Gt(ROSA)26Sor⁺ mice, the mitochondrial network shows initial fragmentation, with (B, F, and H) some large-caliber calbindin-stained dendrites depleted of mitochondria, which appear clustered. Scale bar: 20 um.

Copyright

This image is from Almajan ER, J Clin Invest 2012 Nov 1;122(11):4048-58 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:193564

Associated Alleles

Symbol	Name
Afg3l2^tm1Arte	AFG3-like AAA ATPase 2; targeted mutation 1, TaconicArtemis
Tg(Pcp2-cre)2Mpin	transgene insertion 2, Max-Planck-Institute of Neurobiology

Associated Genotypes

Allelic Composition	Genetic Background
Afg3l2^tm1Arte/Afg3l2^tm1Arte Gt(ROSA)26Sor^tm1Lrsn/Gt(ROSA)26Sor^+ Tg(Pcp2-cre)2Mpin/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6