Phenotype Image Detail

Image

Caption

Evolution of mitochondrial morphology phenotype in Afg3l2^tm1Arte/Afg3l2^tm1Arte Tg(Pcp2-cre)2Mpin/0 Gt(ROSA)26Sor^tm1Lrsn/Gt(ROSA)26Sor⁺ (Afg312^PC-KO) mice. The mitochondrial network is labeled with mt-YFP (B, E, H, K), while Purkinje cell (PC) dendrites are decorated using an antibody against calbindin (A, D, G, J). In merged images (C, F, I, L) pseudocolors were used: calbindin is in green, while mt-YFP is in red. mt-YFP expression in control mice at 4 and 6 weeks of age shows an elongated network of mitochondria (B, H) that follows the pattern of PC dendrites labeled with calbindin (A, C, G, I). In 4 weeks old mutant mice, the mitochondrial network shows initial signs of fragmentation (E, F). At 6 weeks mutant mice display severe fragmentation of the mitochondrial network (K, L). Scale bar 50 um.

Copyright

This image is from Almajan ER, J Clin Invest 2012 Nov 1;122(11):4048-58 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:193564

Associated Alleles

Symbol	Name
Afg3l2^tm1Arte	AFG3-like AAA ATPase 2; targeted mutation 1, TaconicArtemis
Gt(ROSA)26Sor^tm1Lrsn	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Nils-Goran Larsson
Tg(Pcp2-cre)2Mpin	transgene insertion 2, Max-Planck-Institute of Neurobiology

Associated Genotypes

Allelic Composition	Genetic Background
Afg3l2^tm1Arte/Afg3l2^tm1Arte Gt(ROSA)26Sor^tm1Lrsn/Gt(ROSA)26Sor^+ Tg(Pcp2-cre)2Mpin/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6