Phenotype Image Detail

Image

Caption

Photoreceptor and retinal pigmented epithelium (RPE) structures in wild-type (WT), Rho^tm2.1Kpal/Rho⁺ (EK) and Rho^tm2.1Kpal/Rho^tm2.1Kpal (KK) mice. (A) Plastic sections of P30 KK mouse retinas stained with toluidine blue show disorganized photoreceptor outer segment (OS) with dying photoreceptor cells (arrows). Scale bar: 20 um. (B) Transmission electron microscopy (TEM) images depict disordered photoreceptor OS discs in P30 KK mouse retina but not in retinas from WT or EK littermates. In WT and EK retinas, higher magnification images reveal normal rod outer segments (ROS) architecture, whereas in the KK retina, there is severe ROS disorganization characterized by ROS with loosened and irregular shaped discs together with inclusions (arrows). Scale bars: 1 um. (C) Phagosomes in P14 WT, EK, and KK RPE. Normal phagosomes were observed in P14 WT and EK mouse RPE (boxes with dashed lines in the upper two rows; scale bar: 4 um). Augmented phagosome images are shown in right panels. Scale bar, 1 um. P14 KK mouse retinas display severe RPE pathology with undigested phagocytized discs (arrows, bottom panels) and even ingested discs show abnormal processing. (D) TEM images of retinas from 4-month-old WT, EK, and KK mice. Compared with WT retinas, EK retinas exhibit largely normal-appearing discs in most regions of the outer segment, with just a few abnormal deposits (*) in the subretinal space. KK mouse retinas completely lacked photoreceptor disc structures at this age (&) but pigmented deposits (#) are seen in their subretinal space. Scale bar: 5 um. IS, photoreceptor inner segment.

Copyright

This image is from Zhang N, J Clin Invest 2013 Jan 2;123(1):121-37 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:194158

Associated Alleles

Symbol	Name
Rho^tm2.1Kpal	rhodopsin; targeted mutation 2.1, Krzysztof Palczewski

Associated Genotypes

Allelic Composition	Genetic Background
Rho^tm2.1Kpal/Rho^+	involves: 129S6/SvEvTac * C57BL/6J * C57BL/6NTac
Rho^tm2.1Kpal/Rho^tm2.1Kpal	involves: 129S6/SvEvTac * C57BL/6J * C57BL/6NTac