Phenotype Image Detail

Image

Caption

Rhodopsin mislocalization, fundus autofluorescent spots, and Muller/macrophage/microglial cell activation in retinas of Rho^tm2.1Kpal/Rho^tm2.1Kpal (KK) mice. (A) E150K rhodopsin probed with 1D4 antibody mislocalized in part to the inner segment and perinuclear region of 1-month-old mouse retina. In constrast, rhodopsin localized normally within the rod outer segments (ROS) of wild-type (WT) and heterozygous (EK) retina. (B) Scanning laser ophthalmoscopy (SLO) fundus images from WT, EK, and KK mice at 2 months of age. Numerous autofluorescent spots are evenly distributed throughout the KK mouse fundus, whereas autofluorescence is barely visible in WT fundus. EK fundus exhibited a moderate fluorescent phenotype. (C) Immunohistochemistry of 1-month-old WT, EK, and KK mouse retinas with GFAP antibody that labels activated Muller cells. Massive activation of Muller cells is evident in KK but not in WT or EK retina. (D) Immunohistochemical analysis of 1-month-old WT, EK, and KK mouse retinas with Iba1 antibody (green) used to mark macrophage or microglia. In WT and EK retinas, Iba1 immunoreactivity was observed only in the ganglion cell layer (GCL) to the outer plexiform layer (OPL), whereas in KK retina, Iba1 labeling also localized to the outer photoreceptor cell layer (arrows). DAPI was used to counterstain nuclei. All immunohistochemistry was done with retinas from 1 month-old mice. Scale bars: 40 um. IS, photoreceptor inner segment.

Copyright

This image is from Zhang N, J Clin Invest 2013 Jan 2;123(1):121-37 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:194158

Associated Alleles

Symbol	Name
Rho^tm2.1Kpal	rhodopsin; targeted mutation 2.1, Krzysztof Palczewski

Associated Genotypes

Allelic Composition	Genetic Background
Rho^tm2.1Kpal/Rho^tm2.1Kpal	involves: 129S6/SvEvTac * C57BL/6J * C57BL/6NTac