Phenotype Image Detail

Image

Caption

Expansion of the subventricular zone in Gt(ROSA)26Sor^{tm1(RICTOR)Jger}/Gt(ROSA)26Sor^{tm1(RICTOR)Jger} Tg(GFAP-cre)25Mes/0 and Gt(ROSA)26Sor^{tm1(RICTOR)Jger}/Gt(ROSA)26Sor^{tm1(RICTOR)Jger} Tg(GFAP-cre)25Mes/0 Tg(GFAP-EGFR*,-lacZ)#Agu/0 mice. (A) Parasagittal sections of the subventricular zone (SVZ) from control, Gt(ROSA)26Sor^{tm1(RICTOR)Jger}/Gt(ROSA)26Sor^{tm1(RICTOR)Jger} Tg(GFAP-cre)25Mes/0 (Rictor) and Gt(ROSA)26Sor^{tm1(RICTOR)Jger}/Gt(ROSA)26Sor^{tm1(RICTOR)Jger} Tg(GFAP-cre)25Mes/0 Tg(GFAP-EGFR*,-lacZ)#Agu/0 (EGFRvIII x Rictor) mice. Arrows show the SVZ/rostral migratory stream in mature control mouse brain; ST, striatum; CC, corpus callosum; V; ventricle. Scale bar, 100 um. (B) The SVZ was measured at its single widest point (left panel, representative measurement; arrow) and quantified. Five mice of each strain were examined. (C) Expanded SVZ in Rictor mice are composed of distinct cell populations. Representative adjacent sections stained with H&E, immunohistochemically with anti-TuJ1 and anti-GFAP antibodies as indicated. Similar results were observed in SVZ sections in EGFRvIII x Rictor mice. (D) Representative SVZ sections stained immunohistochemically with anti-Ki-67 and anti-phospho-S473-AKT antibodies from Rictor and Rictor x EGFRvIII mice as indicated. In B-D, scale bar; 20 um. (E) Quantification of phospho-S473-AKT immunopositive cell numbers in the SVZ of control, Rictor and Rictor x EGFRvIII mice. A representative count in triplicate out of three individual counts with similar results is shown with + S.D.

Copyright

This image is from Bashir T, PLoS One 2012;7(10):e47741, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:192206

Associated Alleles

Symbol	Name
Gt(ROSA)26Sor^tm1(RICTOR)Jger	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Joseph Gera
Tg(GFAP-EGFR*,-lacZ)#Agu	transgene insertion, Abhijit Guha

Associated Genotypes

Allelic Composition	Genetic Background
Gt(ROSA)26Sor^tm1(RICTOR)Jger/Gt(ROSA)26Sor^tm1(RICTOR)Jger Tg(GFAP-cre)25Mes/0	involves: 129 * FVB/N
Gt(ROSA)26Sor^tm1(RICTOR)Jger/Gt(ROSA)26Sor^tm1(RICTOR)Jger Tg(GFAP-cre)25Mes/0 Tg(GFAP-EGFR*,-lacZ)#Agu/0	involves: 129S1/Sv * 129X1/SvJ * FVB/N