Phenotype Image Detail

Image

Caption

Blastocysts that are Uso1^{Gt(YTA025)Byg}/Uso1^{Gt(YTA025)Byg} (YTA025) or Uso1^{Gt(AW0562)Wtsi}/Uso1^{Gt(AW0562)Wtsi} (AW0562) have a dispersed Golgi architecture. Confocal laser scanning double immunofluorescence images (magnification 400x) of cells within cultured E3.5 blastocysts that were recovered from heterozygous Uso1 gene trapped mating pairs. Antibodies recognizing epitopes in the USO1 carboxyl-terminal domain (red fluorescence) or the Golgi protein GM130 (green fluorescence) were used. DAPI staining was used to mark cell nuclei (blue fluorescence). In cells from blastocysts containing immuno-detectable USO1, GM130 localizes near the cell nuclei, overlapping with USO1 protein, GM130 does not localize near the nucleus but is more dispersed throughout the cytoplasm.

Copyright

This image is from Kim S, PLoS One 2012;7(11):e50530, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:195576

Associated Alleles

Symbol	Name
Uso1^Gt(AW0562)Wtsi	USO1 vesicle docking factor; gene trap AW0562, Wellcome Trust Sanger Institute
Uso1^Gt(YTA025)Byg	USO1 vesicle docking factor; gene trap YTA025, BayGenomics

Associated Genotypes

Allelic Composition	Genetic Background
Uso1^Gt(AW0562)Wtsi/Uso1^Gt(AW0562)Wtsi	involves: 129P2/OlaHsd * C57BL/6
Uso1^Gt(YTA025)Byg/Uso1^Gt(YTA025)Byg	involves: 129P2/OlaHsd * C57BL/6