Phenotype Image Detail

Image

Caption

Scanning electron micrographs of inner (IHC) and outer (OHC) hair cells from 8 week old mice. (A-D) wild-type (Myo7a+/+), (E-L) Myo7a^ewaso/Myo7a^ewaso (Myo7a^I487N/I487N) and (M-T) Myo7a^dmbo2/Myo7a^dmbo2 (Myo7a^F947I/F947I) mice at the middle and basal cochlear level. OHC bundles of Myo7a^ewaso/Myo7a^ewaso mutants appear to have a regular V shaped array, however are missing the majority of their inner-row stereocilia at the middle level (arrow head in E). This is more severe at the basal level where many OHC bundles are missing (G). This phenotype is more severe in Myo7a^dmbo2/Myo7a^dmbo2 mutants, where OHC bundles are disoriented, forming an array of OHC-type bundles (M, Q, O and S). Many Myo7a^ewaso/Myo7a^ewaso IHC bundles are affected at this age, with less numbers of stereocilia within the bundle and those remaining often showing signs of fusion (asterisk in J). The IHC bundles in Myo7a^dmbo2/Myo7a^dmbo2 contain additional rows of stereocilia, however do still maintain a staircase-like structure (N, R, P and T). Scale bar; 2 uM.

Copyright

This image is from Miller KA, PLoS One 2012;7(12):e51284, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:195666

Associated Alleles

Symbol	Name
Myo7a^dmbo2	myosin VIIA; dumbo2
Myo7a^ewaso	myosin VIIA; ewaso

Associated Genotypes

Allelic Composition	Genetic Background
Myo7a^ewaso/Myo7a^ewaso	involves: C57BL/6J
Myo7a^dmbo2/Myo7a^dmbo2	involves: C57BL/6J