Phenotype Image Detail

Image

Caption

The Dscam^3J mutation reproduces some aspects of the Dscam null retina. A-C, Retinal sections from wild-type (Dscam^+/+), Dscam^2J/Dscam^2J (Dscam^2J/2J) and Dscam^3J/Dscam^3J (Dscam^3J/3J) mice were stained with hematoxylin and eosin. In the wild-type retina (A) the three cellular layers are neatly stacked and the synapse containing plexiform layers do not intrude within the cellular layers. B, Cell number is increased in the Dscam^2J/Dscam^2J retina, with ectopic cells located in the inner plexiform layer, which projects into the inner nuclear layer. C, The Dscam^3J/Dscam^3J retina is hypercellular; however, cellular lamination is more neatly organized compared to the Dscam^2J/Dscam^2J retina. D-F, Retinal ganglion cell spacing and arborization is disrupted in both the Dscam^2J/Dscam^2J and Dscam^3J/Dscam^3J retina compared to wild-type. G-I, Amacrine cell spacing and arborization is disrupted in the Dscam^2J/Dscam^2J retina but this degree of disruption is not observed in the Dscam^3J/Dscam^3J retina (I). J, Occasional loose fasciculation of Dscam^3J/Dscam^3J dopaminergic cell neurites was observed (arrows). K and L, Wild-type and Dscam^3J/Dscam^3J retinas were stained with antibodies to bNOS, to detect bNOS-positive amacrine cells. The number of bNOS positive amacrine cells is increased in the Dscam^3J/Dscam^3J retina. The scale bar in (A-C) is equivalent to 132 um. The scale bar in (I) is equivalent to 320 um in D-I and K and L, and 100 um in J.

Copyright

This image is from Schramm RD, PLoS One 2012;7(12):e52652, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:195831

Associated Alleles

Symbol	Name
Dscam^2J	DS cell adhesion molecule; 2 Jackson
Dscam^3J	DS cell adhesion molecule; 3 Jackson

Associated Genotypes

Allelic Composition	Genetic Background
Dscam^3J/Dscam^3J	involves: BALB/c * C3H/HeSnSmn * C57BL/Ka
Dscam^2J/Dscam^2J	involves: C3H/HeDiSnJ