Phenotype Image Detail

Image

Caption

Endogenous Gja3 influences cataract formation caused by the Gja8^R205G mutation. (A) Lens photos of wild-type (Gja8^+/+ Gja3^+/+), Gja8^R205G/Gja8⁺ (Gja8^R205G/+Gja3^+/+), Gja8^R205G/Gja8^R205G (Gja8^R205G/R205GGja3^+/+), and double mutant Gja8^R205G/Gja8^R205G Gja3^tm1Scr/Gja3^tm1Scr (Gja8^R205G/R205GGja3^-/-) mice at the age of 3 weeks. These mutant lines were bred into the C57BL/6J strain background. While the Gja8^R205G/Gja8^R205G lens revealed vacuole-like defects in the lens periphery (indicated by a white arrow), the double Gja8^R205G/Gja8^R205G Gja3^tm1Scr/Gja3^tm1Scr mutant lens showed a clear lens periphery (indicated by a white arrow). Scale bar, 1 mm. (B) Histological sections of Gja8^R205G/Gja8^R205G mutant and Gja8^R205G/Gja8^R205G Gja3^tm1Scr/Gja3^tm1Scr mutant lenses from 3-week-old littermates. The homozygous Gja8^R205G/Gja8^R205G lens section showed disorganized peripheral fibers with vacuoles or enlarged extracellular spaces (labeled with asterisk) near the lens bow region (indicated by an arrowhead), while the double Gja8^R205G/Gja8^R205G Gja3^tm1Scr/Gja3^tm1Scr mutant lens section shows organized and elongated periphery fiber cells at the bow region (indicated by an arrowhead). Scale bar, 50 um.

Copyright

This image is from Xia CH, PLoS One 2012;7(12):e52894, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:195727

Associated Alleles

Symbol	Name
Gja3^tm1Scr	gap junction protein, alpha 3; targeted mutation 1, The Scripps Research Institute
Gja8^R205G	gap junction protein, alpha 8; Arg205Gly

Associated Genotypes

Allelic Composition	Genetic Background
Gja8^R205G/Gja8^R205G	B6.Cg-Gja8^R205G
Gja3^tm1Scr/Gja3^tm1Scr Gja8^R205G/Gja8^R205G	involves: 129S4/SvJae * CWD/LeAgl * SJL/J