Phenotype Image Detail

Image

Caption

Yap1 is required for kidney development. (A) Stages of nephrogenesis and their relationship to the ureteric bud (UB, black) tips. Signals released from UB tips induce mesenchyme cells to condense around UB tips forming the cap mesenchyme (CM, blue). Some of these CM cells aggregate forming the pretubular aggregate (PA) that converts into epithelial renal vesicle (RV). The late RV fuses with UB tips and develops into comma (CSB) and S-shaped (SSB) body. (A') Schematic diagram of the nephron components. (B) Confocal images for Yap1, E-cadherin and DAPI staining in late RV at E14.5. Nuclear Yap1 is observed in the proximal segment of the RV (arrowheads), while Yap1 expression disappears in Six2:Cre expressing cells (D - arrows point to CM cells, arrowhead points to an early nephron). (C) Confocal images of p-Yap1/E-cadherin/DAPI staining shows ubiquitous p-Yap1 expression. (E) Immunohistochemistry using Yap1/Taz antibody in RV and SSB shows a similar expression pattern observed with Yap1 antibody in previous panels (arrowheads). (F-F") Confocal images for Yap1/E-cadherin/DAPI staining in SSB at E14.5. Nuclear Yap1 is observed in proximal and distal segments of the SSB (arrowheads). (G,H) Macroscopic view of the urogenital system from wild-type and Yap1^tm1Hmc/Yap1^tm1Hmc Six2^{tm1(tTA,tetO-EGFP/cre)Amc}/Six2⁺ (Yap^CM-/-) kidneys at P0. Note bilateral reduction in kidney size of mutant compared to control and empty bladder in mutant animals. (I,J) PAS staining of P0 kidneys from wild-type and mutant animals. Arrows point to the papilla. (K,L) Closer view of the cortical zone shows limited nephrogenesis in mutants. (M,N) Higher magnification shows abnormal glomeruli structure and tubules with barely discernable lumens (asterisk) in mutants. k: kidney; b: bladder; cd: collectiong duct; csb: comma-shaped body; d: distal; g: glomeruli; ic: inner cortex; ma: medulla; m: medial; nz: nephrogenic zone; p: proximal; pt: proximal tubule; ssb: Sshaped body. Scale bars represent 25 um (B-F"; M-N), 1 um (G-J), 200 um (K,L).

Copyright

This image is from Reginensi A, PLoS Genet 2013 Mar;9(3):e1003380, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:195266

Associated Alleles

Symbol	Name
Six2^tm1(tTA,tetO-EGFP/cre)Amc	sine oculis-related homeobox 2; targeted mutation 1, Andrew P McMahon
Yap1^tm1Hmc	yes-associated protein 1; targeted mutation 1, Helen McNeill

Associated Genotypes

Allelic Composition	Genetic Background
Yap1^tm1Hmc/Yap1^tm1Hmc Six2^tm1(tTA,tetO-EGFP/cre)Amc/Six2^+	involves: 129 * 129S6/SvEvTac * C57BL/6J * C57BL/6NCrl