Phenotype Image Detail

Image

Caption

Yap1 deletion impairs nephron induction, without affecting self-renewal of the cap mesenchyme (CM) population. (A,B) Immunostaining analysis for Six2 (E14.5) shows no change in expression pattern in both genotypes (arrows). E-cadherin was used to visualize the ureteric bud (UB) compartment. (C,D) Dramatic reduction in nephrogenesis visualized by loss of NCAM-expressing structures (arrowheads) in the nephrogenic zone of mutant compared to wild-type (E16.5). Note the reduced NCAM expression in CM cells. Calbindin highlights the UB and collecting duct (CD). (E) Quantification of early nephron structures in E15.5 controls (black columns) and mutants (white columns) based on NCAM staining. Total***: p<0.0001; PA***:p<0.0001; RV*: p = 0.0209; CSB*: p = 0.0018; SSB***: p,0.0001. (F,G) ISH analysis shows maintained Gdnf expression in CM of control and mutants (E15.5). (H,I) WT1 staining (E18.5) reveals staining in CM cells (arrows) for both genotypes, and dramatic reduction in number of renal mesenchyme-to-epithelial transition (MET)-derived structures in mutants compared to wild-type. (J,K) Immunostaining analysis for the CM marker Sall1 (E14.5) shows no change in expression pattern in both genotypes. E-cadherin was used to visualize the UB compartment. Scale bars represent 100 um.

Copyright

This image is from Reginensi A, PLoS Genet 2013 Mar;9(3):e1003380, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:195266

Associated Alleles

Symbol	Name
Six2^tm1(tTA,tetO-EGFP/cre)Amc	sine oculis-related homeobox 2; targeted mutation 1, Andrew P McMahon
Yap1^tm1Hmc	yes-associated protein 1; targeted mutation 1, Helen McNeill

Associated Genotypes

Allelic Composition	Genetic Background
Yap1^tm1Hmc/Yap1^tm1Hmc Six2^tm1(tTA,tetO-EGFP/cre)Amc/Six2^+	involves: 129 * 129S6/SvEvTac * C57BL/6J * C57BL/6NCrl