Phenotype Image Detail

Image

Caption

Loss of Cdc42 phenocopies loss of Yap1. (A,B) PAS staining of E14.5 wild-type and Cdc42^tm1Brak/Cdc42^tm1Brak Six2^{tm1(tTA,tetO-EGFP/cre)Amc}/Six2⁺ (Cdc42^CM-/-) kidneys showing the presence of condensing mesenchymal cells (arrows) but dramatic loss of cap mesenchyme (CM)-derived epithelial structures (arrowheads). Immunostaining analysis for Six2 (C,D), Sall1 (E,F), and WTl (G,H) at E14.5 shows presence of CM cells in both genotypes (arrows). E-cadherin and Calbindin were used to visualize the ureteric bud (UB) compartment. (I,J) Immunostaining at E14.5 shows normal expression of Pax2 in the UB, but decreased expression in the mutant CM cells (arrows). (K,L) In situ hybridization at E14.5 reveals decrease in Uncx4.1 in mutant. Scale bars represent 100 um.

Copyright

This image is from Reginensi A, PLoS Genet 2013 Mar;9(3):e1003380, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:195266

Associated Alleles

Symbol	Name
Cdc42^tm1Brak	cell division cycle 42; targeted mutation 1, Cord Brakebusch
Six2^tm1(tTA,tetO-EGFP/cre)Amc	sine oculis-related homeobox 2; targeted mutation 1, Andrew P McMahon

Associated Genotypes

Allelic Composition	Genetic Background
Cdc42^tm1Brak/Cdc42^tm1Brak Six2^tm1(tTA,tetO-EGFP/cre)Amc/Six2^+	involves: 129 * C57BL/6J