Phenotype Image Detail

Image

Caption

Alteration of skin structure at the earlier stages of Slc27a4^pskn/Slc27a4^pskn (mutant) mice. Hair follicle induction was assayed using a BMP4-lacZ reporter line from E14.5 to E16.5. Embryos were incubated in solution with 5-bromo-4-chloro-3-indolyl-b- D galactoside (X-gal). X-gal cleavage by beta-galactosidase results in dark blue staining. A, At E14.5, representative control and mutant embryos display blue-stained primary hair follicles (PHFs) and vibrissal follicles. There were similar numbers of PHFs in the lateral side of control and mutant mice. B, Peeled skin from representative control and mutant E16.5 embryos was stained with X-gal. PHFs are larger and often show an unstained core and a distinctive ring shape (red arrows). Secondary hair follicles (SHFs) are smaller and are more numerous. The ratio of SHFs to PHFs in the mutant epidermis is significantly decreased compared to that of the control (n = 3, p<0.01). C, D. Intact E15.5 control embryos display bluestained hair follicles over most of their surface, except for local regions on the dorsum with limited staining (black arrow) (C). In contrast, mutant embryos had large portions of their back and lateral skin as well as ventral sites (white arrow) that were not stained by X-gal, indicating alternated permeability at E15.5 (D).

Copyright

This image is from Tao J, PLoS One 2012;7(11):e50634, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:194779

Associated Alleles

Symbol	Name
Slc27a4^pskn	solute carrier family 27 (fatty acid transporter), member 4; pigskin

Associated Genotypes

Allelic Composition	Genetic Background
Slc27a4^pskn/Slc27a4^pskn	involves: FVB