Phenotype Image Detail

Image

Caption

Impaired migration and differentiation of enteric ganglion progenitors in Phox2b^tm1.1Heno/Phox2b⁺ (Phox2b^del5/+) and Phox2b^tm2.1Heno/Phox2b⁺ (Phox2b^del8/+) embryos. (A-C) In situ hybridization (ISH) analysis of Sox10 expression in enteric neural crest-derived cells (ENCCs). Arrows indicate the foreguts of boxed E10.5 embryos. Insets in the upper-right corners are larger magnifications of boxed areas. (D-F and H-J) Phox2b/Sox10 double immunostaining of E12 gut. Invasion of ENCCs in the hindgut mesenchyme (D) is retarded in Phox2b mutant embryos (E and F). ENCC density was noticeably lower in the mutant than in wild-type (WT) midgut. (G) Schematic representation showing the gut morphology at E12. Areas covered by ENCCs in WT hindgut are shown in green. (H-J) Larger magnification of the midgut regions. Arrows indicate neuronally committed cells expressing WT Phox2b, but not Sox10. (K-P) TuJ1 staining of embryonic gut. Tuj1+ cells are drastically decreased in mutant embryos (L and M). Extinguishment of Sox10 expression in these early differentiating neurons is also impaired in mutant embryos (arrows in O and P). Scale bars: 10 um (P); 20 um (J); 200 um (C, F and M).

Copyright

This image is from Nagashimada M, J Clin Invest 2012 Sep 4;122(9):3145-58 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:190742

Associated Alleles

Symbol	Name
Phox2b^tm1.1Heno	paired-like homeobox 2b; targeted mutation 1.1, Hideki Enomoto
Phox2b^tm2.1Heno	paired-like homeobox 2b; targeted mutation 2.1, Hideki Enomoto

Associated Genotypes

Allelic Composition	Genetic Background
Phox2b^tm1.1Heno/Phox2b^+	either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * C57BL/6 * DBA/2)
Phox2b^tm2.1Heno/Phox2b^+	either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * C57BL/6 * DBA/2)