Phenotype Image Detail

Image

Caption

Abnormal neuroglial differentiation of sympathetic ganglion cells in Phox2b^tm1.1Heno/Phox2b⁺ (Phox2b^del5/+) and Phox2b^tm2.1Heno/Phox2b⁺ (Phox2b^del8/+) embryos. (A) Expression of wild-type (WT) Phox2b and Sox10 in the mascent sympathetic ganglia. Note that Sox10+Phox2b- cells (white arrows) are more prominent in mutant sympathetic gnaglia. (B) Expression of various markers in the sympathetic chain. First and second rows represent TH staining of whole-mount preparations and transverse sections of E10.5 embryos. The third through sixth rows show results of in situ hybridization (ISH) analysis. (C and E) Analysis of neuroglial differentiation in developing sympathetic ganglia by Phox2b/Sox10 immunohistochemistry. Although Sox10+ cells (satellite cell progenitors) are localized preferentially at the periphery of the WT ganglia at E11.5 (C, white arrowheads), many Sox10+ cells are also found inside of the sympathetic ganglia in mutant embryos (C, arrows). (D) BrdU (red) and WT Phox2b (green) double labeling of the E11.5 sympathetic chain ganglia. (E) Note that, at E18.5, Sox10+ cells appear more prominent in mutant than in WT sympathetic ganglia (SCG). Scale bars: 20 um (A, C-E); 150 um (B, TH, Dbh, Ret, Ascl1, Erbb3); 500 um (B, TH, whole-mount).

Copyright

This image is from Nagashimada M, J Clin Invest 2012 Sep 4;122(9):3145-58 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:190742

Associated Alleles

Symbol	Name
Phox2b^tm1.1Heno	paired-like homeobox 2b; targeted mutation 1.1, Hideki Enomoto
Phox2b^tm2.1Heno	paired-like homeobox 2b; targeted mutation 2.1, Hideki Enomoto

Associated Genotypes

Allelic Composition	Genetic Background
Phox2b^tm1.1Heno/Phox2b^+	either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * C57BL/6 * DBA/2)
Phox2b^tm2.1Heno/Phox2b^+	either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * C57BL/6 * DBA/2)