Phenotype Image Detail

Image

Caption

Characterization of Mob1a^tm1.1Asuz/Mob1a^tm1.1Asuz Tg(KRT14-cre/ERT)20Efu/0 Mob1b^{Gt(CC0690)Wtsi}/Mob1b^{Gt(CC0690)Wtsi}(DKO) mice. (A-C) Features of 16-day-old mutant mice that were originally left untreated (control) or treated with tamoxifen (kDKO) at P1. (A) kDKO(P1) mice have a wrinkled-bear facial appearance (top left), ruffled, shaggy body hair (bottom left), abnormally large front paws (top right), and decreased survival (bottom right). (B) H&E-stained longitudinal and transverse sections of the back skin of the mice in A. kDKO(P1) mice show hyperplasia of interfollicular epidermis (IFE) and hair follicles (HFs). Scale bars: 50 um. (C) kDKO(P1) mice show parakeratosis (reduced enucleation) in stratum corneum layers. Scale bar: 20 um. (D) Immunohistochemical analyses of fat pad epidermis of the mice in A using Alexa Fluor 488-tagged Abs recognizing the indicated differentiation markers corresponding to a specific skin layer. DAPI, nuclei. Scale bar: 50 um. (E) Immunohistochemical analyses of back skin of the mice in A using Abs recognizing the indicated skin layer markers. For top panels, cells were stained with Alexa Fluor 568-tagged anti-KRT14 Ab. DAPI, nuclei. Scale bars: 20 um. For bottom panels, cells were stained with Alexa Fluor 488-tagged anti-Trichohyalin Ab and Fluor 568-tagged anti-KRT17 Ab. Scale bar: 50 um. (F) H&E-stained longitudinal sections of skin from 42-day-old Mob1a^tm1.1Asuz/Mob1a^tm1.1Asuz Tg(KRT14-cre/ERT)20Efu/0 Mob1b^{Gt(CC0690)Wtsi}/Mob1b^{Gt(CC0690)Wtsi} mice that were left untreated (control) or treated with tamoxifen (kDKO) at P28. The kDKO (P28) mutant shows hyperplasia of IFE and HFs. Scale bar: 200 um. Results shown are representative of at least 3 independent trials and at least 3 mice/group. Data are presented as the mean +/- SEM.

Copyright

This image is from Nishio M, J Clin Invest 2012 Dec 3;122(12):4505-18 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:193981

Associated Alleles

Symbol	Name
Mob1a^tm1.1Asuz	MOB kinase activator 1A; targeted mutation 1.1, Akira Suzuki
Mob1b^Gt(CC0690)Wtsi	MOB kinase activator 1B; gene trap CC0690, Wellcome Trust Sanger Institute
Tg(KRT14-cre/ERT)20Efu	transgene insertion 20, Elaine Fuchs
Tg(KRT14-cre/ERT)20Efu	transgene insertion 20, Elaine Fuchs

Associated Genotypes

Allelic Composition	Genetic Background
Mob1a^tm1.1Asuz/Mob1a^tm1.1Asuz Mob1b^Gt(CC0690)Wtsi/Mob1b^Gt(CC0690)Wtsi Tg(KRT14-cre/ERT)20Efu/0	involves: 129P2/OlaHsd * C57BL/6 * CD-1