Phenotype Image Detail

Image

Caption

Kif7 is necessary for the patterning, differentiation, and induction of Scleraxis (Scx)-positive tendon progenitors in the central tendon. (A-D) Whole-mount immunohistochemical-stained E17.5 wild-type (WT) and Kif7^dda/Kif7^dda (Kif7^dd/dd) diaphragms. Mutants show loss of tenascin C (Tnc) expression at the myotendonous junction (MTJ) (arrowheads in A and B) and an overgrowth of muscle fibers (arrowheads in C and D). (E and F) MyHC (green) and tenomodulin (Tnmd; red) coimmunofluorescent staining in sagittal sections of the anterior diaphragm at the plane marked by dashed line in C and D in E17.5 control and mutant embryos. Insets show a zoomed-in view of the MTJ in WT and mutant diaphragms (arrowheads). (G and H) Col1a1 whole-mount in situ hybridization at E15.5. (I and J) Col1a1 immunofluorescent staining in frontal sections of the diaphragm at the plane marked by the dashed line in G and H in E15.5 control and mutant embryos. (K-P) Scx whole-mount in situ hybridization at E12.5, E13.5, and E14.5 in WT and mutant diaphragms. Arrowheads show reduced induction of Scx in the central tendon domain of the diaphragm in mutant embryos relative to littermate controls.

Copyright

This image is from Coles GL, Proc Natl Acad Sci U S A 2013 May 21;110(21):E1898-905. Copyright 2013 National Academy of Sciences, U.S.A. J:197449

Associated Alleles

Symbol	Name
Kif7^dda	kinesin family member 7; disorganized diaphragm allele

Associated Genotypes

Allelic Composition	Genetic Background
Kif7^dda/Kif7^dda	FVB.A-Kif7^dda