Phenotype Image Detail

Image

Caption

Tg(Col1a1-cre)1Bek/0 Pkd1^tm2Ggg/Pkd1^tm2Ggg (Col1a1(3.6)-Cre;Pkd1^flox/flox) mice show enhanced adipogenesis in bone marrow and in bone stromal cell cultures (A) Histology of adipocytes in decalcified tibias. Oil Red O staining (upper panel) showed that the numbers of adipocytes and fat droplets in tibia bone marrow were greater in 6-week-old Tg(Col1a1-cre)1Bek/0 Pkd1^tm2Ggg/Pkd1^tm2Ggg mice compared with age-matched control Pkd1^tm2Ggg/Pkd1^tm2Ggg (Pkd1^flox/flox) mice. Osmium tetroxide (OsO4) staining by microCT analyses (lower panel) showed that adipocyte volume/marrow volume (Ad.V/Ma.V, %) and adipocyte number (Ad.N, mm^-3) were much higher in the proximal tibia from 6-week-old Tg(Col1a1-cre)1Bek/0 Pkd1^tm2Ggg/Pkd1^tm2Ggg mice compared with age-matched control Pkd1^tm2Ggg/Pkd1^tm2Ggg mice. (B) Adipocytic differentiation in BMSC cultures. An increase of adipogenesis potential was observed in 6-week-old Tg(Col1a1-cre)1Bek/0 Pkd1^tm2Ggg/Pkd1^tm2Ggg BMSC cultures, evidenced by a significant increase of Oil Red O staining in adipogenic cultures. Data are expressed as the mean +/- S.D. from three independent experiments. #Significant difference from control (Pkd1^flox/flox) at P<0.05.

Copyright

This image is from Qiu N, PLoS One 2012;7(9):e46038, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:191967

Associated Alleles

Symbol	Name
Pkd1^tm2Ggg	polycystin 1, transient receptor potential channel interacting; targeted mutation 2, Gregory G Germino
Tg(Col1a1-cre)1Bek	transgene insertion 1, Barbara E Kream

Associated Genotypes

Allelic Composition	Genetic Background
Pkd1^tm2Ggg/Pkd1^tm2Ggg Tg(Col1a1-cre)1Bek/0	involves: 129S4/SvJae * CD-1