Phenotype Image Detail

Image

Caption

Col1a1(3.6)-Cre mediated conditional deletion of Pkd1 causes polycystic pancreas and kidney. (A) Gross appearance of liver, kidney, and pancreas. There was no cyst formation in the liver, kidney, and pancreas in Pkd1^tm2Ggg/Pkd1⁺ Tg(Col1a1-cre)1Bek/0 (Col1a1(3.6)-Cre;Pkd1^flox/+) (heterozygous) mice, whereas age matched Pkd1^tm2Ggg/Pkd1^tm2Ggg Tg(Col1a1-cre)1Bek/0 (Col1a1(3.6)-Cre;Pkd1^flox/flox) (homozygous) mice developed severe renal and pancreatic cysts at 6 weeks of age. (B-D) Hematoxylin-eosin stained sections (5X) of liver, pancreas, and kidney from 6-week-old mice. Cysts were not observed in the livers from heterozygous and homozygous mice, and renal and pancreatic cysts were also not found in kidney and pancreas tissues from heterozygous mice. However, homozygous mice exhibited massive cyst formation in both the pancreas and kidney. Interestingly, glomeruli formation in the kidney and endocrine islet formation in pancreas appeared to be unaffected. In addition, expansion of pancreatic ducts formed large pancreatic cysts that led to massive acinar cell loss, formation of abnormal tubular structures, and appearance of endocrine cells in ducts.

Copyright

This image is from Qiu N, PLoS One 2012;7(9):e46038, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:191967

Associated Alleles

Symbol	Name
Pkd1^tm2Ggg	polycystin 1, transient receptor potential channel interacting; targeted mutation 2, Gregory G Germino
Tg(Col1a1-cre)1Bek	transgene insertion 1, Barbara E Kream

Associated Genotypes

Allelic Composition	Genetic Background
Pkd1^tm2Ggg/Pkd1^tm2Ggg Tg(Col1a1-cre)1Bek/0	involves: 129S4/SvJae * CD-1