Phenotype Image Detail

Image

Caption

Aortic root atherosclerosis. A) Representative trichrome-stained images of lesions in the aortic root and lesion area measurements. Left image: Apoe^tm1Unc/Apoe^tm1Unc (11betaHSD1^+/+/ApoE^-/-) aortic root. Right image: Apoe^tm1Unc/Apoe^tm1Unc Hsd11b1^tm1Lex/Hsd11b1^tm1Lex (11betaHSD1^-/-/ApoE^-/-) aortic root. Asterisks denote regions of acellular necrotic debris. Plot:Lesion area measurements from trichrome-stained images in Apoe^tm1Unc/Apoe^tm1Unc (n = 10) and Apoe^tm1Unc/Apoe^tm1Unc Hsd11b1^tm1Lex/Hsd11b1^tm1Lex (n = 9) male mice. B) Intraplaque necrotic areas. Left image: Apoe^tm1Unc/Apoe^tm1Unc aortic root plaque. Right image: Apoe^tm1Unc/Apoe^tm1Unc Hsd11b1^tm1Lex/Hsd11b1^tm1Lex aortic root plaque. Asterisks denote necrotic areas within lesions. Acellularity and features of crystalline clefts were used to demarcate necrotic regions. Plot: Necrotic core area measurements from trichrome-stained images in Apoe^tm1Unc/Apoe^tm1Unc (n = 10) and Apoe^tm1Unc/Apoe^tm1Unc Hsd11b1^tm1Lex/Hsd11b1^tm1Lex (n = 9) male mice. C) Macrophage staining via CD68 immunohistochemistry. Left image: Apoe^tm1Unc/Apoe^tm1Unc aortic root. Right image: Apoe^tm1Unc/Apoe^tm1Unc Hsd11b1^tm1Lex/Hsd11b1^tm1Lex aortic root. Arrows denote CD68 positive staining. Plot: CD68 positive stained area measurements in Apoe^tm1Unc/Apoe^tm1Unc (n - 10) and Apoe^tm1Unc/Apoe^tm1Unc Hsd11b1^tm1Lex/Hsd11b1^tm1Lex (n = 9) male mice. Significance vs. control: *p<= 0.05. Data are pooled from two independently run in vivo studies.

Copyright

This image is from Garcia RA, PLoS One 2013;8(2):e53192, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:199430

Associated Alleles

Symbol	Name
Apoe^tm1Unc	apolipoprotein E; targeted mutation 1, University of North Carolina
Hsd11b1^tm1Lex	hydroxysteroid 11-beta dehydrogenase 1; targeted mutation 1, Lexicon Genetics

Associated Genotypes

Allelic Composition	Genetic Background
Apoe^tm1Unc/Apoe^tm1Unc Hsd11b1^tm1Lex/Hsd11b1^tm1Lex	B6.129-Hsd11b1^tm1Lex Apoe^tm1Unc