Phenotype Image Detail

Image

Caption

Abnormal airway capillary coupling. (A and B) IHC for PECAM an endothelial marker, showing defect in capillary formation at E15.5 in Pten^tm1Hwu/Pten^tm1Hwu Twist2^{tm1.1(cre)Dor}/Twist2⁺ (Pten^{fl/fl;Dermo-Cre}) mice. (C and D) Transmission electron microscopy confirmed increased distance (white arrows) between capillaries (red asterisks: blood cells) and alveoli in Pten^tm1Hwu/Pten^tm1Hwu Twist2^{tm1.1(cre)Dor}/Twist2⁺ lungs. (E) The airway-capillary distance was significantly thicker in mutant lungs (*P < 0.01, n = 3). Scale bars: 50 um (A and B); 5 um (C and D).

Copyright

This image is from Tiozzo C, J Clin Invest 2012 Nov 1;122(11):3862-72 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:192736

Associated Alleles

Symbol	Name
Pten^tm1Hwu	phosphatase and tensin homolog; targeted mutation 1, Hong Wu
Twist2^tm1.1(cre)Dor	twist basic helix-loop-helix transcription factor 2; targeted mutation 1.1, David M Ornitz

Associated Genotypes

Allelic Composition	Genetic Background
Pten^tm1Hwu/Pten^tm1Hwu Twist2^tm1.1(cre)Dor/Twist2^+	B6.129-Twist2^tm1.1(cre)Dor Pten^tm1Hwu