Phenotype Image Detail

Image

Caption

Defect in angioblast differentiation in Pten^tm1Hwu/Pten^tm1Hwu Twist2^{tm1.1(cre)Dor}/Twist2⁺ Kdr^tm1Jrt/Kdr⁺ (Pten^{fl/fl;Dermo-Cre} Flk1^LacZ) lungs showing increased Kdr (Flk1)-positive cells, a marker for angioblasts in the mutant lungs. (B and C) Whole mount Beta-gal staining of the left lobe. Increased Kdr (Flk1)-positive cells in the mutant lungs compared with control was observed. (D and E) Double staining showed majority of Kdr (Flk1)-positive cells are PECAM (a marker for mature endothelial cells) negative. Scale bars: 25 um (lower magnification), 50 um (higher magnification) (B-E).

Copyright

This image is from Tiozzo C, J Clin Invest 2012 Nov 1;122(11):3862-72 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:192736

Associated Alleles

Symbol	Name
Kdr^tm1Jrt	kinase insert domain protein receptor; targeted mutation 1, Janet Rossant
Pten^tm1Hwu	phosphatase and tensin homolog; targeted mutation 1, Hong Wu
Twist2^tm1.1(cre)Dor	twist basic helix-loop-helix transcription factor 2; targeted mutation 1.1, David M Ornitz

Associated Genotypes

Allelic Composition	Genetic Background
Kdr^tm1Jrt/Kdr^+ Pten^tm1Hwu/Pten^tm1Hwu Twist2^tm1.1(cre)Dor/Twist2^+	B6.129-Twist2^tm1.1(cre)Dor Kdr^tm1Jrt Pten^tm1Hwu