Phenotype Image Detail

Image

Caption

(A-D) Diffuse pattern of Prdm1 expression in E6.5 (B) and E7.0 (D) Wnt3^tm1Brd/Wnt3^tm1Brd (Wnt3^-/-) embryos and absence of the cluster of Prdm1-positive primordial germ cell (PGC) progenitors compared with that in Wnt3^tm1Brd/Wnt3⁺ (Wnt3^+/-) (A, C) counterparts. (E-J) Absence of PGCs that were marked by AP (E, F), Dppa3 (G, H) and Ifitm3 (I, J) in Wnt3^tm1Brd/Wnt3^tm1Brd embryos compared with that in Wnt3^tm1Brd/Wnt3⁺ embryos (arrowhead: PGCs). (E, F, I, J) E7.75 and (G, H) E8.0 embryos. (K-M') Reduced number of Dppa3-expressing PGCs in Ctdnep1^tm1Ryn/Ctdnep1⁺ Wnt3^tm1Brd/Wnt3⁺ (Dullard^+/-; Wnt3^+/-) embryos. (K-M) Bright-field images of E7.75 (K) Wnt3^tm1Brd/Wnt3⁺ and (L, M) Ctdnep1^tm1Ryn/Ctdnep1⁺ Wnt3^tm1Brd/Wnt3⁺ embryos (posterior view). (K'-M') Dppa3/Pgc7/Stella immunostaining of PGCs in the posterior region of embryos shown in K-M. Scale bars = 100 um.

Copyright

This image is from Tanaka SS, PLoS One 2013;8(3):e57428, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:199855

Associated Alleles

Symbol	Name
Ctdnep1^tm1Ryn	CTD nuclear envelope phosphatase 1; targeted mutation 1, Ryuichi Nishinakamura
Wnt3^tm1Brd	wingless-type MMTV integration site family, member 3; targeted mutation 1, Allan Bradley

Associated Genotypes

Allelic Composition	Genetic Background
Ctdnep1^tm1Ryn/Ctdnep1^+ Wnt3^tm1Brd/Wnt3^+	involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6